Low-resolution molecular shape, biochemical characterization and emulsification properties of a halotolerant esterase from Bacillus licheniformis.

European Biophysics Journal : EBJ
Ana Elisa T LeiteIgor Polikarpov

Abstract

Bacterial esterases are highly versatile enzymes, currently widely used in detergents, biosurfactants, bioemulsifiers and as biocatalysts in paper and food industries. Present work describes heterologous expression, purification, and biophysical and biochemical characterization of a halotolerant esterase from Bacillus licheniformis (BlEstA). BlEstA preferentially cleaves pNP-octanoate and both activity and stability of the enzyme increased in the presence of 2 M NaCl, and also with several organic solvents (ethanol, methanol and DMSO). Furthermore, BlEstA has considerable emulsifying properties, particularly with olive oil as substrate. Our studies also show that the enzyme is monomeric in solution and its small-angle X-ray scattering low-resolution molecular envelope fits well its high-resolution homology model.

References

Nov 22, 1996·Journal of Molecular Biology·J PontiusS J Wodak
May 11, 2000·Extremophiles : Life Under Extreme Conditions·D MadernG Zaccai
Jun 16, 2000·Applied Microbiology and Biotechnology·I M BanatS S Cameotra
May 24, 2001·Biophysical Journal·D I SvergunM H Koch
Oct 11, 2003·Biotechnology Advances·R SharmaU C Banerjee
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Mar 31, 2004·Canadian Journal of Microbiology·Marcus SchallmeyOwen P Ward
Feb 1, 1987·Applied and Environmental Microbiology·D G Cooper, B G Goldenberg
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Jun 24, 2008·Biotechnology Advances·Babu JosephGeorge Thomas
Aug 30, 2008·Bioresource Technology·Sebastián TorresGuillermo R Castro
Feb 10, 2009·Applied Biochemistry and Biotechnology·Sanghamitra Dutta, Lalitagauri Ray
Jan 1, 2008·Analytical Chemistry Insights·John Geraldine Sandana Mala, Satoru Takeuchi
Nov 3, 2011·Current Protein & Peptide Science·Veronique Receveur-Brechot, Dominique Durand
Dec 24, 2011·BMC Structural Biology·Alessandro SiglioccoloStefano Pascarella
Apr 19, 2012·Biology Direct·Grzegorz M BoratynThomas L Madden
Dec 1, 2012·Enzyme Research·Anuradha BalanFatimah Azzahra Ahmad Rashid
Jan 1, 2012·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Limpon Bora, Minakshi Bora
Jul 1, 1996·World Journal of Microbiology & Biotechnology·H Khyami-Horani
Apr 1, 2014·Protein Expression and Purification·Cesar M Camilo, Igor Polikarpov
Apr 8, 2014·International Journal of Biological Macromolecules·Lavanya Balaji, Gurunathan Jayaraman
May 7, 2014·Enzyme Research·Deepthy AlexRajeev K Sukumaran
Jun 28, 2014·Biomolecules·Jose L Adrio, Arnold L Demain
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Dec 23, 2015·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Palanisamy KanmaniJeyaseelan Aravind
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Dec 10, 2016·Frontiers in Microbiology·Sanket J JoshiMusallam S Al-Mandhari
Dec 16, 2016·Journal of Chemical Information and Modeling·Yu-Feng LinChih-Hao Lu
Mar 16, 2017·Acta Crystallographica. Section D, Structural Biology·Heping ZhengWladek Minor
Feb 9, 2018·International Journal of Biological Macromolecules·Weijia ZhangYi Cao
May 23, 2018·Nucleic Acids Research·Andrew WaterhouseTorsten Schwede
Jul 18, 2018·Extremophiles : Life Under Extreme Conditions·Patricia S KumagaiAna P U Araujo
Oct 30, 2018·Protein Science : a Publication of the Protein Society·Vassili PiiadovIgor Polikarpov

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Datasets Mentioned

BETA
AAU39460.1

Methods Mentioned

BETA
biosensor
size-exclusion chromatography
X-ray
thermal shift
circular dichroism

Software Mentioned

CheckMyMetal
SAVES
Gnom
MIB ( Metal Ion - Binding site prediction and docking server )
MUSCLE
WHATCHECK
MEGA7
VERIFY3D
PROCHECK
Bio

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