Low shear stress induced HMGB1 translocation and release via PECAM-1/PARP-1 pathway to induce inflammation response

PloS One
Wei-dong QinMingxiang Zhang

Abstract

Low shear stress (LSS) plays a critical role in the site predilection of atherosclerosis through activation of cellular mechanosensors, such as platelet endothelial cell adhesion molecule 1 (PECAM-1). Poly(ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that regulates the expression of various inflammatory cytokines. The nuclear enzyme high mobility group box 1 (HMGB1) can induce inflammation response by binding to toll-like receptor 4 (TLR4). In the present study, we aimed to investigate the role and mechanism of HMGB1 in LSS induced inflammation in human umbilical vein endothelial cells (HUVECs). HUVECs were stimulated by undisturbed shear stress (USS, 1 Pa) and LSS (0.4 Pa) in our experiments. Gene expression was inhibited by small interfering RNA (siRNA). ICAM-1 expression was regulated by LSS in a time dependent manner. LSS can induce HMGB1 translocation from nucleus to cytoplasm and release. Compared with the USS, LSS could increase the protein expression of PECAM-1 and PARP-1 as well as the secretion of TNF-α and IL-1β. LSS induced the translocation of HMGB1 from nucleus to cytoplasm. Inhibition of HGMB1 reduced LSS-induced inflammatory response. Inhibition of PARP-1 suppressed inflammatory response through inhibit...Continue Reading

References

Feb 24, 1989·Science·M E BianchiG Paonessa
Sep 13, 1969·Nature·C G CaroR C Schroter
Nov 1, 1984·Biophysical Chemistry·M Cohen-Armon
Jan 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·P L WalpolaB L Langille
Nov 1, 1993·Journal of Biomechanical Engineering·S Weinbaum, S Chien
Sep 6, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Bürkle
Jul 12, 2002·Nature·Paola ScaffidiMarco E Bianchi
Nov 21, 2002·Cellular and Molecular Life Sciences : CMLS·P O Hassa, M O Hottiger
Mar 7, 2003·American Journal of Physiology. Cell Physiology·Jong Sung ParkEdward Abraham
Sep 6, 2003·American Journal of Physiology. Heart and Circulatory Physiology·J Scott McNallyDavid G Harrison
Jun 24, 2004·The Journal of Pharmacology and Experimental Therapeutics·Pál PacherCsaba Szabó
Apr 2, 2005·Nature Reviews. Immunology·Michael T Lotze, Kevin J Tracey
May 3, 2005·Nature Reviews. Drug Discovery·Prakash Jagtap, Csaba Szabó
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ingrid E DumitriuPatrizia Rovere-Querini
Sep 6, 2005·Genes & Development·Mi Young KimW Lee Kraus
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefania MitolaMarco Presta
Aug 4, 2007·Circulation Research·Ravi M RaoFrancis W Luscinskas
Aug 4, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Elena Galkina, Klaus Ley
Feb 26, 2008·Intensive Care Medicine·Sari KarlssonEsko Ruokonen
Mar 5, 2008·Circulation·Robert E PoelmannBeerend P Hierck
Dec 10, 2008·The Journal of Experimental Medicine·Vilma UrbonaviciuteReinhard E Voll
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Huan YangKevin J Tracey

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Citations

Jan 16, 2018·Oxidative Medicine and Cellular Longevity·Zhilin QiQiang Li
Feb 16, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Yeshuo MaAlex F Chen
Jul 17, 2020·Journal of the American Heart Association·Robert P HebbelAmir Lerman
May 26, 2018·Journal of Biomechanical Engineering·Ashkan JavadzadeganAndy S C Yong
Jul 22, 2018·BMC Complementary and Alternative Medicine·Ming ZhangRuomei Qi
Sep 7, 2019·Journal of Cellular Physiology·Jia LiuNanping Wang
Sep 18, 2018·Molecular Medicine Reports·András VidaPéter Bai
Mar 19, 2019·Computer Methods in Biomechanics and Biomedical Engineering·Ashkan JavadzadeganHamid Hassanzadeh Afrouzi

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Methods Mentioned

BETA
transfection
PCR
confocal microscopy
ELISA

Software Mentioned

SPSS
Image
Pro Plus

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