Low Shear Stress Upregulates CX3CR1 Expression by Inducing VCAM-1 via the NF-κB Pathway in Vascular Endothelial Cells.

Cell Biochemistry and Biophysics
Yiwei ZhaoYongmei Nie

Abstract

Atherosclerosis is a significant cause of mortality and morbidity. Studies suggest that the chemokine receptor CX3CR1 plays a critical role in atherogenesis. Shear stress is an important mechanical force that affects blood vessel function. In this study, we investigated the effect of shear stress on CX3CR1 expression in vascular endothelial cells (VECs). First, cells were exposed to different shear stress and then CX3CR1 mRNA and protein were measured by quantitative RT-PCR and western blot analysis, respectively. CX3CR1 gene silencing was used to analyze the molecular mechanisms underlying shear stress-mediated effects on CX3CR1 expression. CX3CR1 mRNA and protein expression were significantly increased with 4.14 dyne/cm2 of shear stress compared with other tested levels of shear stress. We observed a significant increase in CX3CR1 mRNA levels at 2 h and CX3CR1 protein expression at 4 h. CX3CR1-induced VCAM-1 expression in response to low shear stress by activating NF-κB signaling pathway in VECs. Our findings demonstrate that low shear stress increases CX3CR1 expression, which increases VCAM-1 expression due to elevated NF-κB activation. The current study provides evidence of the correlation between shear stress and atheroscl...Continue Reading

References

Feb 13, 1997·Nature·J F BazanT J Schall
May 23, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·O Traub, B C Berk
Nov 14, 2000·Circulation Research·H Cai, D G Harrison
Feb 6, 2003·The Journal of Clinical Investigation·Philippe LesnikIsrael F Charo
May 7, 2003·Progress in Biophysics and Molecular Biology·Nitzan ResnickEfrat Wofovitz
Dec 18, 2007·Endothelium : Journal of Endothelial Cell Research·Min ChengHuaiqing Chen
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Cornelia Hahn, Martin A Schwartz
Nov 6, 2010·Antioxidants & Redox Signaling·Patrizia NigroBradford C Berk
Apr 16, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hong Liu, Deqian Jiang
Aug 17, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Peter Libby
Jul 28, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Lucie PoupelChristophe Combadiere
Aug 27, 2013·Acta Pharmacologica Sinica·Stavros Apostolakis, Demetrios Spandidos
Nov 6, 2013·Journal of Cell Science·Daniel E Conway, Martin A Schwartz
Jan 30, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Joseph W WraggRoy Bicknell
May 31, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Jing ZhouShu Chien
Sep 19, 2014·European Heart Journal·Brenda R KwakPaul C Evans
Aug 2, 2015·Advances in Clinical Chemistry·Xiao-Hua YuChao-Ke Tang
Dec 18, 2015·Thrombosis and Haemostasis·Stephanie Lehoux, Elizabeth A Jones
May 20, 2017·Mediators of Inflammation·Aaron BabendreyerAndreas Ludwig
Feb 7, 2018·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Amanguli RuzeYongmei Nie
Apr 5, 2018·International Journal of Molecular Sciences·Deok-Hoon KongSukmook Lee
Apr 12, 2018·PloS One·Jimmy Jianheng ZhouAnne M Durkan

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Citations

Dec 19, 2020·Frontiers in Cardiovascular Medicine·Aaron BabendreyerAndreas Ludwig
Aug 6, 2021·Journal of Inflammation Research·Shafiu A Umar ShingeFengxu Yu

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Methods Mentioned

BETA
PCR
Protein Assay
flow cytometry
transfection

Software Mentioned

SPSS

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