Low source-sink ratio reduces reserve starch in grapevine woody canes and modulates sugar transport and metabolism at transcriptional and enzyme activity levels

Planta
Angélica SilvaHernâni Gerós

Abstract

Severe leaf removal decreases storage starch and sucrose in grapevine cv. Cabernet Sauvignon fruiting cuttings and modulates the activity of key enzymes and the expression of sugar transporter genes. Leaf removal is an agricultural practice that has been shown to modify vineyard efficiency and grape and wine composition. In this study, we took advantage of the ability to precisely control the number of leaves to fruits in Cabernet Sauvignon fruiting cuttings to study the effect of source-sink ratios (2 (2L), 6 (6L) and 12 (12) leaves per cluster) on starch metabolism and accumulation. Starch concentration was significantly higher in canes from 6L (42.13 ± 1.44 mg g DW-1) and 12L (43.50 ± 2.85 mg g DW-1) than in 2L (22.72 ± 3.10 mg g DW-1) plants. Moreover, carbon limitation promoted a transcriptional adjustment of genes involved in starch metabolism in grapevine woody tissues, including a decrease in the expression of the plastidic glucose-6-phosphate translocator, VvGPT1. Contrarily, the transcript levels of the gene coding the catalytic subunit VvAGPB1 of the VvAGPase complex were higher in canes from 2L plants than in 6L and 12L, which positively correlated with the biochemical activity of this enzyme. Sucrose concentration ...Continue Reading

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Citations

Jul 20, 2018·Planta·Henrique NoronhaHernâni Gerós
Mar 1, 2020·Ecological Applications : a Publication of the Ecological Society of America·Zihe ZhangWei Zhang

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Methods Mentioned

BETA
PCR
PCRs
enzymatic assay

Software Mentioned

PRANKSTER
PROTDIST
Genedoc
RETREE
BLAST
Prism
Mega
NEIGHBOR
PHYLIP

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