Low temperature-induced variation in plasma biochemical indices and aquaglyceroporin gene expression in the large yellow croaker Larimichthys crocea

Scientific Reports
Cheng LiuJun-Quan Zhu

Abstract

Low temperature influences multiple physiological processes in fish. To explore the adaptability of the large yellow croaker (Larimichthys crocea) to low temperature, the concentrations of glycerol, blood urea nitrogen (BUN), and triglycerides (TG) in plasma, as well as the expression levels of metabolism-related genes aqp7 and aqp10, were measured after exposure to low temperature stress and during subsequent rewarming. In addition, tissue samples from the intestine and liver were histologically analyzed. We found that the concentrations of plasma glycerol, BUN, and TG, decreased under low temperature stress, suggesting the metabolism of fat and protein slowed at low temperature. The expression levels of aqp7 and aqp10 mRNA were also downregulated under exposure to low temperature. Interestingly, above plasma indices and gene expression returned to basic levels within 24 h after rewarming. Furthermore, the liver and the intestine were damaged under continuous low temperature stress, whereas they were repaired upon rewarming. From the above results, we concluded that aqp7 and aqp10 genes were sensitive to low temperature, and that the decrease in their expression levels at low temperature might reduce energy consumption by L. c...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·E C Lin
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 24, 2002·Current Opinion in Clinical Nutrition and Metabolic Care·Daniel C Baumgart, Axel U Dignass
Nov 25, 2003·FEBS Letters·Peter Agre, David Kozono
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Landon S KingPeter Agre
Jul 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Toshiyuki HibuseIichiro Shimomura
Aug 5, 2005·Journal of Cell Science·A S Verkman
Aug 10, 2005·The American Journal of Gastroenterology·Ashkan FarhadiJeremy Z Fields
Aug 22, 2006·FEBS Letters·Amaia RodríguezGema Frühbeck
Jul 27, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Carissa M Krane, David L Goldstein
Oct 27, 2007·Annual Review of Physiology·Aleksandra RojekRobert A Fenton
Mar 20, 2009·Cardiovascular Research·Toshiyuki HibuseIichiro Shimomura
May 12, 2009·Cardiovascular Research·Monika GladkaPaula A da Costa Martins
Mar 10, 2010·The International Journal of Biochemistry & Cell Biology·Ying LuuSamuel B Ho
Apr 23, 2010·The Journal of Biological Chemistry·Dao-Yao HeDorit Ron
May 28, 2013·Developmental and Comparative Immunology·Giuseppe Scapigliati
Jan 28, 2014·Journal of Molecular Endocrinology·Janne Lebeck
Mar 15, 2014·Nature Reviews. Drug Discovery·Alan S VerkmanMarios C Papadopoulos
Jul 11, 2014·Molecular and Cellular Endocrinology·Leire Méndez-GiménezGema Frühbeck
May 20, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jennifer R HallWilliam R Driedzic
Jan 5, 2018·International Journal of Molecular Sciences·Francesco Maria Iena, Janne Lebeck

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