Low template STR typing: effect of replicate number and consensus method on genotyping reliability and DNA database search results

Forensic Science International. Genetics
Corina BenschopTitia Sijen

Abstract

To analyze DNA samples with very low DNA concentrations, various methods have been developed that sensitize short tandem repeat (STR) typing. Sensitized DNA typing is accompanied by stochastic amplification effects, such as allele drop-outs and drop-ins. Therefore low template (LT) DNA profiles are interpreted with care. One can either try to infer the genotype by a consensus method that uses alleles confirmed in replicate analyses, or one can use a statistical model to evaluate the strength of the evidence in a direct comparison with a known DNA profile. In this study we focused on the first strategy and we show that the procedure by which the consensus profile is assembled will affect genotyping reliability. In order to gain insight in the roles of replicate number and requested level of reproducibility, we generated six independent amplifications of samples of known donors. The LT methods included both increased cycling and enhanced capillary electrophoresis (CE) injection [1]. Consensus profiles were assembled from two to six of the replications using four methods: composite (include all alleles), n-1 (include alleles detected in all but one replicate), n/2 (include alleles detected in at least half of the replicates) and 2...Continue Reading

References

Aug 15, 1996·Nucleic Acids Research·P TaberletJ Bouvet
Jul 7, 2000·Forensic Science International : Synergy·P GillJ Buckleton
Sep 22, 2005·Forensic Science International : Synergy·John Buckleton, Christopher Triggs
Oct 26, 2005·The Journal of Law, Medicine & Ethics : a Journal of the American Society of Law, Medicine & Ethics·Robin Williams, Paul Johnson
Jun 9, 2006·Forensic Science International : Synergy·Peter GillJames Curran
Dec 17, 2008·Forensic Science International. Genetics·Peter GillJim Lambert
Feb 14, 2009·Forensic Science International. Genetics·Peter GillJames Curran
May 8, 2009·Science & Justice : Journal of the Forensic Science Society·Andrew J GibbRosalind M Brown
May 30, 2009·Croatian Medical Journal·Bruce BudowleAngela van Daal
Aug 4, 2009·Forensic Science International. Genetics·Torben TvedebrinkNiels Morling
Aug 4, 2009·Forensic Science International. Genetics·John Buckleton
Dec 2, 2009·Forensic Science International. Genetics·David J Balding, John Buckleton
Dec 19, 2009·PloS One·Mark W Perlin, Alexander Sinelnikov
May 12, 2010·Forensic Science International. Genetics·Peter Gill, John Buckleton

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Citations

Dec 2, 2011·International Journal of Legal Medicine·Corina BenschopTitia Sijen
Dec 10, 2013·Forensic Science International. Genetics·Vince L Pascali, Sara Merigioli
Dec 3, 2010·Investigative Genetics·Roland Ah van OorschotR John Mitchell
May 14, 2014·International Journal of Molecular Medicine·Na HuChunling Ma
Oct 8, 2013·International Journal of Legal Medicine·Jianye Ge, Bruce Budowle
Jan 12, 2013·International Journal of Legal Medicine·Antoinette A WestenTitia Sijen
Mar 26, 2013·Forensic Science International. Genetics·Claus BørstingNiels Morling
Jun 29, 2012·Forensic Science International. Genetics·Céline M PfeiferPeter Wiegand
May 29, 2012·Forensic Science International. Genetics·Antoinette A WestenTitia Sijen
May 23, 2012·Forensic Science International. Genetics·Corina C G BenschopTitia Sijen
Dec 14, 2011·Forensic Science International. Genetics·C SchneiderB Siebertz
Aug 16, 2011·Forensic Science International. Genetics·Jo-Anne BrightJohn Buckleton
Apr 2, 2011·Forensic Science International. Genetics·Natalie E C WeilerTitia Sijen
Dec 17, 2010·Forensic Science International. Genetics·Peter M SchneiderAngel Carracedo
Mar 14, 2016·Forensic Science International. Genetics·Peter Gill
Jul 24, 2015·Forensic Science International. Genetics·Corina C G BenschopTitia Sijen
Sep 20, 2015·Science & Justice : Journal of the Forensic Science Society·Corina C G BenschopTitia Sijen
Dec 9, 2014·Forensic Science International. Genetics·Hinda HanedTitia Sijen
Feb 21, 2014·Forensic Science International. Genetics·M van den BergeT Sijen
Jun 24, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John M Butler
Feb 18, 2014·Forensic Science International. Genetics·S GittelsonF Taroni
May 1, 2016·Forensic Science International : Synergy·Simone GittelsonMichael D Coble
Oct 6, 2014·Forensic Science International. Genetics·Ronny HedellJohannes Hedman
Dec 19, 2012·Forensic Science International. Genetics·P Gill, H Haned
Apr 22, 2014·Forensic Science International. Genetics·Corina C G Benschop, Titia Sijen
Oct 23, 2016·Forensic Science International. Genetics·Alycia K BuckinghamRoland A H van Oorschot
Mar 20, 2014·BioTechniques·Kelly Grisedale, Angela van Daal
Jul 4, 2019·International Journal of Legal Medicine·Elena I ZavalaMitchell M Holland
May 31, 2018·International Journal of Legal Medicine·Michelle HarrelSheree Hughes-Stamm
Apr 11, 2018·Journal of Forensic Sciences·Mian Sahib ZarGalina Kulstein
Dec 29, 2020·Science & Justice : Journal of the Forensic Science Society·Kaitlin HuffmanJack Ballantyne
Nov 2, 2017·Forensic Science International. Genetics·Lauren E AlfonseCatherine M Grgicak
Sep 3, 2021·Forensic Science International. Genetics·Max SchmidtVolker Weirich

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