PMID: 9425128Feb 28, 1998Paper

Low toxicity of nitric oxide against endothelial cells under physiological oxygen partial pressures

The Biochemical Journal
I IoannidisHerbert de Groot

Abstract

Cultured rat liver endothelial cells were incubated with 1 and 2 mM spermineNONOate at different O2 concentrations in the incubation atmosphere. (Z)-1-{N-[3-Aminopropyl]-N-[4-(3-aminopropylammonio) butyl]-amino}diazen-1-ium-1,2- diolate (spermineNONOate), at 2 mM, was highly cytotoxic at 21% and 95% O2 (as measured by lactate dehydrogenase release); more than 80% of the cells were damaged after 6 h of incubation. Cytotoxicity induced by 2 mM spermineNONOate was significantly decreased at 10%, 5% and 0% O2; cell death was 54%, 36% and 25% respectively after 6 h of incubation. In contrast, 1 mM spermineNONOate was almost non-toxic towards the cells. Only at 95% O2 was a slight damaging effect, of 25%, observed. The nitric oxide (.NO) concentrations released from 1 and 2 mM spermineNONOate were determined as varying between 5 and 12 microM and between 12 and 22 microM respectively as measured by the oxyhaemoglobin and the NO cheletropic spin-trapping methods. The decomposition rate of spermineNONOate and the resulting .NO concentrations were independent of O2 at all applied concentrations. Likewise, the steady-state concentrations of H2O2 remained at approx. 1 nM at all O2 concentrations, as measured with the fluorescent dye scopo...Continue Reading

Citations

Jan 12, 2002·British Journal of Pharmacology·Yinke YangJames P Morgan
Jun 27, 2006·Life Sciences·Frank PetratUrsula Rauen
Oct 23, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·T KiviluotoE Kivilaakso
May 13, 1999·Biochimica Et Biophysica Acta·G C Brown

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