Low tristetraprolin expression promotes cell proliferation and predicts poor patients outcome in pancreatic cancer

Oncotarget
Zi-Ran WeiQing-Ping Cai

Abstract

Tristetraprolin (also known as TTP, TIS11, ZFP36, and Nup475) is a well-characterized tumor suppressor that is down-regulated in several tumor types. In the current study, we found that TTP expression was markedly reduced in pancreatic cancer samples as compared to matched normal tissues. Low TTP level was associated with age (P=0.037), tumor size (P=0.008), tumor differentiation (P=0.004), postoperative T stage (pT stage, P<0.001), postoperative N stage (pN stage, P=0.008) and TNM stage (P<0.001). Moreover, low TTP expression predicted reduced survival rates and poor patient outcome. We also found that TTP impairs pancreatic cancer cell proliferation both in vivo and in vitro. Fluorescence Activated Cell Sorting (FACS) assay showed that TTP over-expression both increases apoptosis and decreases proliferation in pancreatic cancer cells. RNA-sequencing analysis showed that TTP over-expression downregulates several tumor-related factors, including Pim-1 and IL-6. Our findings indicate that TTP could serve as a potential prognostic indicator in pancreatic cancer.

References

Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Mar 20, 2001·Gene·J Guhaniyogi, G Brewer
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eeva-Marja RainioPäivi J Koskinen
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
Apr 30, 2005·Journal of Cell Science·Lynne E Maquat
Aug 12, 2005·Cancer Immunology, Immunotherapy : CII·Graziella BelloneGiorgio Emanuelli
Mar 21, 2006·Journal of Autoimmunity·Danielle Mercatante CarrickPerry J Blackshear
Aug 8, 2006·Biochemical Pharmacology·Bharat B AggarwalGautam Sethi
Aug 19, 2008·Cancer Biology & Therapy·Carolin Reiser-ErkanJörg Kleeff
Jun 17, 2009·The American Journal of Pathology·Jian ChenMong-Hong Lee
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Jun 16, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelElizabeth Ward
Aug 7, 2012·Cell·Robert J RounbehlerJohn L Cleveland
Sep 22, 2012·Biochemical and Biophysical Research Communications·Xia ChenQingping Cai
Jan 26, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elizabeth A Van TubergenNisha J D'Silva
Aug 8, 2014·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Reinhild HolmerHolger Kalthoff
Dec 30, 2014·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Soyeong KangYung-Jue Bang
Mar 6, 2015·World Journal of Gastroenterology : WJG·Ya-Jun ChengLiang-Hua Wang
Nov 6, 2015·Molecules and Cells·Jinhyun RyuSang Soo Kang

❮ Previous
Next ❯

Citations

Sep 21, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hao WangYulan Ruan
Sep 16, 2017·Acta Biochimica Et Biophysica Sinica·Jin Shang, Zhanzheng Zhao
Nov 11, 2017·Medical Oncology·Jian GuoJiazeng Xia
Feb 24, 2017·Wiley Interdisciplinary Reviews. RNA·Alison Galloway, Martin Turner
Nov 2, 2018·International Journal of Molecular Sciences·Jeong-Min ParkTae-Hong Kang
Jul 2, 2020·World Journal of Clinical Cases·Li-Yan WangJian-Bin Zhang
Aug 16, 2018·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Robert J RounbehlerKosj Yamoah
Feb 10, 2021·Journal of Cellular Physiology·Amirreza BitarafSadegh Babashah
Apr 3, 2021·Molecular Genetics and Metabolism·Gabriel Rodríguez-GómezAlfonso León-Del-Río

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
xenografts
FACS
PCR
protein assay
RNA-seq

Software Mentioned

Quantity One

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.