Abstract
Cell lysis, where cellular material is released, is the basis for the separation and purification of cell contents, biochemical analysis, and other related experiments. It is also a key step in molecular, real-time, and cancer diagnoses as well as in the drug screening of pathogens. The current methods of lysing cells have several limitations, such as damage to the activity of cellular components, the need for a large number of cell samples, time-consuming processes, and the danger of high voltage. Therefore, a simple, fast, and efficient method for the manipulation of micro-volume cells or for single cell lysis is significant for further scientific research and practical application. In this study, a new low-voltage controllable method for cell lysis was established, and a corresponding microfluidic chip was developed. Simple, efficient and rapid micro-volume cells and single cell lysis were successfully achieved under a low-voltage alternating current with a voltage of 16 Vp-p and frequency of 10 kHz. The lysis process was investigated in detail by separately labelling the whole cell, cytoplasm, and nucleus using fluorescent proteins, which indicated that the whole cell was completely lysed. Analysis of voltage and frequency ...Continue Reading
References
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K Kinosita, T T Tsong
Aug 1, 1968·Biochimica Et Biophysica Acta·A J Sale, W A Hamilton
Mar 1, 1996·Applied Microbiology and Biotechnology·T Grahl, H Märkl
Mar 10, 2004·Lab on a Chip·Jian GaoZhao-Lun Fang
Apr 22, 2004·Lab on a Chip·S MetzP Renaud
Jun 18, 2010·Lab on a Chip·Guillaume MernierPhilippe Renaud
Jun 1, 2009·Journal of Microelectromechanical Systems : a Joint IEEE and ASME Publication on Microstructures, Microactuators, Microsensors, and Microsystems·Mark J MescherJeffrey T Borenstein
Mar 4, 2011·Biomedical Microdevices·Weijie Wan, John T W Yeow
Oct 26, 2011·Electrophoresis·Amy D HargisJohn Michael Ramsey
Nov 19, 2011·Biomedical Microdevices·Weijie Wan, John T W Yeow
Dec 7, 2013·Molecules and Cells·Morgan Hamon, Jong Wook Hong
Feb 1, 2014·Lab on a Chip·Lang NanXueyong Wei
Apr 4, 2014·Lab on a Chip·Mahla PoudinehShana O Kelley
Jun 27, 2014·Biosensors & Bioelectronics·S Kabiri AmeriS Sonkusale
Dec 17, 2014·Analytical Chemistry·Ali Salehi-ReyhaniDavid R Klug
Citations
Nov 10, 2020·Biomicrofluidics·K PandianA K Sen
May 1, 2021·Micromachines·Emil GrigorovVassil Galabov
May 17, 2021·Food Research International·Xiaoying HanYing Mu
Oct 13, 2021·Journal of Nanobiotechnology·Wen-Min ZhouJian-Ye Zhang