PMID: 8974991Oct 1, 1995Paper

Low-voltage field-emission scanning electron microscopy of non-coated guinea-pig hair cell stereocilia

Hearing Research
E A DunnebierF W Albers

Abstract

The stereociliar structures of the guinea-pig cochlear organ of Corti were studied at low-voltage (1-5 kV) with field-emission scanning electron microscope (SEM) using various pre- and post-fixation methods, such as OTOTO (OsO4/thiocrbohydrazide/OsO4/thiocarbohydrazide/OsO4) and TAO (tannic acid/arginine/OsO4), and different dissection procedures of the cochlea. A perfusion and immersion pre-fixation with glutaraldehyde, in combination with removal of the bony wall and stria vascularis from the cochlea, followed by the TAO non-coating treatment gave the best result at 2 kV acceleration voltage. Due to these new technique, several interesting delicate structures of the stereocilia, in particular fine surface structures, were detected for the first time using SEM. These findings include the different types of cross-links and tip links, i.e., the fine surface morphology of the stereocilia and their attachments and imprints in the tectorial membrane (TM). One of the most interesting findings in this study is a network of long filamentous structures, which has been identified mainly at the top of the longest stereocilia and the undersurface of the TM and which may represent the glycocalyx. These findings and their possible implicati...Continue Reading

References

Jul 1, 1992·Trends in Neurosciences·J O Pickles, D P Corey
Jan 1, 1986·Hearing Research·D J Lim
Jan 1, 1987·Hearing Research·P A Santi, C B Anderson
May 1, 1985·Hearing Research·D N Furness, C M Hackney
Mar 1, 1983·Hearing Research·K P Steel

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Citations

Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Catherine J StarrA J Hudspeth
Dec 18, 2010·The Journal of Comparative Neurology·Elisabeth VerpyChristine Petit
Jun 18, 2016·Microscopy Research and Technique·Magdalena ŻakWilko Grolman
Sep 25, 2001·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·E A DunnebierF W Albers
Jul 31, 2007·Microscopy Research and Technique·Damjana DrobneFrancesco Tatti

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