Low-voltage paper isotachophoresis device for DNA focusing

Lab on a Chip
Xiang LiRichard M Crooks

Abstract

We present a new paper-based isotachophoresis (ITP) device design for focusing DNA samples having lengths ranging from 23 to at least 1517 bp. DNA is concentrated by more than two orders of magnitude within 4 min. The key component of this device is a 2 mm-long, 2 mm-wide circular paper channel formed by concertina folding a paper strip and aligning the circular paper zones on each layer. Due to the short channel length, a high electric field of ~16 kV m(-1) is easily generated in the paper channel using two 9 V batteries. The multilayer architecture also enables convenient reclamation and analysis of the sample after ITP focusing by simply opening the origami paper and cutting out the desired layers. We profiled the electric field in the origami paper channel during ITP experiments using a nonfocusing fluorescent tracer. The result showed that focusing relied on formation and subsequent movement of a sharp electric field boundary between the leading and trailing electrolyte.

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Citations

Oct 9, 2015·Journal of the American Chemical Society·Max M GongDavid Sinton
Mar 18, 2017·Analytical Chemistry·Xiang LiRichard M Crooks
Nov 2, 2017·Nature Communications·Sarah M FriedrichTza-Huei Wang
Feb 2, 2019·Analytical and Bioanalytical Chemistry·Tianyu DongFeng Li
Nov 5, 2019·Electrophoresis·Federico SchaumburgCharles S Henry
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Jan 7, 2020·Electrophoresis·Federico SchaumburgClaudio L A Berli
Jan 13, 2018·Analytical and Bioanalytical Chemistry·Ji-Cheng NiuZhi-Yong Wu
Jul 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Siraprapa BoobphahomKwanwoo Shin
Dec 20, 2018·Lab on a Chip·Tally Rosenfeld, Moran Bercovici
Oct 6, 2017·Journal of Separation Science·Vladimíra DatinskáFrantišek Foret
Aug 30, 2017·Micromachines·Ismail Hussain Kamal BashaNor Hisham Bin Hamid
Jan 6, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Benjamin P SullivanJonathan D Posner
Jun 15, 2021·Chemical Reviews·Eka NovianaCharles S Henry
Apr 7, 2020·Analytical Chemistry·Shakila H Peli ThanthriThomas H Linz
May 16, 2018·Analytical Chemistry·Andrew T BenderJonathan D Posner
Dec 13, 2016·Analytical Chemistry·Yuanyuan YangCharles S Henry

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