PMID: 7541846Aug 1, 1995Paper

Lower in vivo mutation rate of human immunodeficiency virus type 1 than that predicted from the fidelity of purified reverse transcriptase

Journal of Virology
L M Mansky, H M Temin

Abstract

The level of genetic variation of human immunodeficiency virus type 1 (HIV-1), a member of the lentivirus genus of the Retroviridae family, is high relative to that of retroviruses in some other genera. The high error rates of purified HIV-1 reverse transcriptase in cell-free systems suggest an explanation for this high genetic variation. To test whether the in vivo rate of mutation during reverse transcription of HIV-1 is as high as predicted by cell-free studies, and therefore higher than that rates of mutation of retroviruses in other genera, we developed an in vivo assay for detecting forward mutations in HIV-1, using the lacZ alpha peptide gene as a reporter for mutations. This system allows the rates and types of mutations that occur during a single cycle of replication to be studied. We found that the forward mutation rate for HIV-1 was 3.4 x 10(-5) mutations per bp per cycle. Base substitution mutations predominated; G-to-A transition mutations were the most common base substitution. The in vivo mutation rates for HIV-1 are three and seven times higher than those previously reported for two other retroviruses, spleen necrosis virus and bovine leukemia virus, respectively. In contrast, our calculated in vivo mutation rat...Continue Reading

References

Feb 4, 1992·Biochemistry·J P Ji, L A Loeb
May 1, 1992·Journal of Virology·V K Pathak, H M Temin
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J C BoyerT A Kunkel
May 11, 1992·Journal of Virology·G L Buchschacher, A T Panganiban
Nov 30, 1990·Science·W S Hu, H M Temin
Sep 4, 1990·Biochemistry·T A Kunkel
Jan 1, 1990·Annual Review of Genetics·R A Katz, A M Skalka
Nov 1, 1991·AIDS Research and Human Retroviruses·L RatnerD B Trowbridge
Oct 11, 1990·Nature·M Nowak
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W S Hu, H M Temin
Nov 25, 1988·Science·J D RobertsT A Kunkel
Dec 1, 1986·Molecular and Cellular Biology·J P Dougherty, H M Temin
Mar 15, 1968·International Journal of Cancer. Journal International Du Cancer·H M Temin
Jun 27, 1970·Nature·H M Temin, S Mizutani
Jun 1, 1984·Molecular and Cellular Biology·S Kawai, M Nishizawa
Sep 1, 1993·AIDS Research and Human Retroviruses·J E FitzgibbonD T Dubin
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H M Temin
Feb 1, 1993·Journal of Virology·L WillemsR Kettmann
Mar 1, 1993·Journal of Virology·D K DubeJ B Glaser
Mar 1, 1993·Microbiological Reviews·J A Levy

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Citations

Jan 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·E WattelS Wain-Hobson
Mar 1, 1996·AIDS Research and Human Retroviruses·L M Mansky
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·R B MarkhamX F Yu
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·L A LoebJ I Mullins
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·I M Rouzine, J M Coffin
Jun 20, 2001·Journal of Virology·W L Schneider, M J Roossinck
May 9, 2002·Nucleosides, Nucleotides & Nucleic Acids·Robyn M HydeArthur D Broom
Oct 22, 2002·Journal of Virology·Jianling ZhuangJoseph P Dougherty
Jun 29, 2004·Journal of Virology·Cristina PastoreDonald E Mosier
Sep 25, 2004·Bioinformatics·Jinko GrahamFrançoise Seillier-Moiseiwitsch
Nov 17, 2004·The Journal of Experimental Medicine·Nicola A JonesPersephone Borrow
Jul 12, 2005·Antiviral Chemistry & Chemotherapy·Prem L SharmaRaymond F Schinazi
Dec 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Simon D W FrostDouglas D Richman
Mar 7, 2007·Current HIV/AIDS Reports·Kenneth R HenryEric J Arts
Oct 24, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·John M MurrayStefan F Wieland
Jul 17, 2007·PLoS Computational Biology·Marcel E CurlinJohn E Mittler
Nov 8, 2007·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Koen DeforchePhilippe Lemey
Oct 16, 2007·Clinical Microbiology Reviews·Carrie Dykes, Lisa M Demeter
Feb 6, 2009·Journal of Virology·M-R AbrahamsUNKNOWN Center for HIV-AIDS Vaccine Immunology Consortium
Jul 19, 2008·PLoS Computational Biology·Christian L Althaus, Rob J De Boer
May 6, 2010·PLoS Computational Biology·Timothy E SchlubMiles P Davenport
Sep 17, 2010·PloS One·Alicia ArnottUNKNOWN Pulse Study Team
Mar 31, 2010·Acta Microbiologica Et Immunologica Hungarica·Szilvia KanizsaiK Nagy
Oct 22, 2010·AIDS Research and Human Retroviruses·Gustavo H KijakJerome H Kim
Jan 14, 2011·Current Opinion in HIV and AIDS·Frank Maldarelli

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