Lowered Expression of MicroRNAs 221 and 222 Mediate Apoptosis Induced by High Glucose in Human Periodontal Ligament Cells.

Cell Biochemistry and Biophysics
Mariana M MonteiroMarinilce F Santos

Abstract

Impaired periodontal healing is a common complication of diabetes mellitus (DM), frequently related to hyperglycemia. MicroRNAs 221 and 222 have been studied as biomarkers for inflammatory diseases, including diabetes, but their role in the periodontal ligament (PL) is unknown. The effects of high glucose on human PL cells death were studied, as well as the expression of microRNA-221 and microRNA-222, potentially modulated by DM. Cells were obtained from the premolar teeth of young humans and cultured for 7 days under different glucose concentrations (5 or 30 mM). MicroRNAs-221/222 expressions were evaluated by real-time RT-PCR and apoptosis by TUNEL assays. Caspase-3 expression was studied by western blotting and immunocytochemistry. High glucose increased apoptosis and caspase-3 protein expression by about 3×. MicroRNA-221 and microRNA-222 expressions decreased by nearly 40% under high glucose. MicroRNA-221 and microRNA-222 inhibition using antagomiRs increased apoptosis by 2-3×, while the expression of caspase-3, a validated target for these microRNAs, was increased by 50%. The overexpression of both microRNAs using miR mimics in high glucose cells did no effect on apoptosis but increased caspase-3 expression by 30%. In conc...Continue Reading

References

Jul 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Van VlietW Van Ewijk
Feb 1, 1997·Periodontology 2000·W BeertsenJ Sodek
May 10, 2002·Community Dentistry and Oral Epidemiology·Carlene TsaiGeorge W Taylor
Dec 25, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Wojciech J Grzesik, A S Narayanan
Jul 19, 2005·Cancer Research·Jennifer A ChanKenneth S Kosik
Apr 9, 2009·Biochemical and Biophysical Research Communications·Yangxin LiYong-Jian Geng
May 8, 2013·Molecular and Cellular Endocrinology·Chasity B ColemanT Cooper Woods
Jun 26, 2013·Acta Diabetologica·Loredana FiorentinoMassimo Federici
Jun 28, 2014·Molecular Biology Reports·Sercan ErgunSerdar Oztuzcu
Nov 13, 2014·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D X LiJ Ke
Apr 22, 2015·Molecular and Cellular Biochemistry·Bing QinZhengqi Lu
Nov 11, 2015·European Heart Journal·Sarah CostantinoFrancesco Cosentino
Feb 18, 2016·BMC Cancer·Cilene Rebouças LimaMarinilce Fagundes Santos
Jun 17, 2016·Journal of Molecular Biomarkers & Diagnosis·Audrey VillardSophie Rome
Jul 16, 2016·Genetics and Molecular Research : GMR·M Y LiA Y Qiu
Jan 18, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Francesca FornariLaura Gramantieri
Jun 22, 2018·Journal of Periodontology·Maurizio S TonettiKenneth S Kornman
Aug 16, 2018·Nature Reviews. Molecular Cell Biology·Luca F R Gebert, Ian J MacRae
Aug 21, 2018·Frontiers in Endocrinology·Jacob O'BrienChun Peng
Sep 29, 2019·World Journal of Diabetes·Bita Rohani

❮ Previous
Next ❯

Citations

Jun 15, 2021·Seminars in Cell & Developmental Biology·Xianghong LuanThomas G H Diekwisch

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
Assay

Software Mentioned

TARBASE
MICRORNA
Image J
MIRTARBASE
GraphPad Prism

Related Concepts

Related Feeds

Biomarkers for Diabetes

This feed focuses on the latest research on biomarkers used for monitoring disease progression in diabetes.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis