Lowering caveolin-1 expression in human vascular endothelial cells inhibits signal transduction in response to shear stress.

International Journal of Cell Biology
A D van der MeerI Vermes

Abstract

Vascular endothelial cells have an extensive response to physiological levels of shear stress. There is evidence that the protein caveolin-1 is involved in the early phase of this response. In this study, caveolin-1 was downregulated in human endothelial cells by RNAi. When these cells were subjected to a shear stress of 15 dyn/cm(2) for 10 minutes, activation of Akt and ERK1/2 was significantly lower than in control cells. Moreover, activation of Akt and ERK1/2 in response to vascular endothelial growth factor was significantly lower in cells with low levels of caveolin-1. However, activation of integrin-mediated signaling during cell adhesion onto fibronectin was not hampered by lowered caveolin-1 levels. In conclusion, caveolin-1 is an essential component in the response of endothelial cells to shear stress. Furthermore, the results suggest that the role of caveolin-1 in this process lies in facilitating efficient VEGFR2-mediated signaling.

References

Feb 24, 1997·Biochemical and Biophysical Research Communications·D KaiserP Friedl
Oct 3, 1998·The Journal of Biological Chemistry·V RizzoJ E Schnitzer
Nov 7, 1998·The Journal of Biological Chemistry·I S BhullarS Chien
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·S JalaliS Chien
Feb 24, 2001·Basic Research in Cardiology·L RössigA M Zeiher
Mar 14, 2001·American Journal of Physiology. Cell Physiology·J LiuM P Lisanti
Jan 17, 2003·Molecular Biology of the Cell·Lyne LabrecqueRichard Béliveau
Apr 26, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Sivaramaprasad GudiJohn A Frangos
May 24, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Nolan L BoydHanjoong Jo
Jun 21, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Victor RizzoPeter F Davies
Oct 23, 2003·The Journal of Biological Chemistry·Michael E DavisDavid G Harrison
Oct 9, 2004·American Journal of Physiology. Heart and Circulatory Physiology·C Radel, V Rizzo
Aug 9, 2005·Journal of Biomechanics·Yi-Shuan J LiShu Chien
Sep 24, 2005·American Journal of Physiology. Cell Physiology·Eric A OsbornJohn H Hartwig
Dec 19, 2006·Biochemical and Biophysical Research Communications·Hui-Wen SunMei Zhang

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Citations

Jun 11, 2011·Pulmonary Medicine·Rajamma Mathew
Aug 12, 2014·Journal of Cellular Physiology·Luis F de CastroArancha Gortazar
Dec 14, 2011·Free Radical Biology & Medicine·Peter R Kvietys, D Neil Granger
Mar 15, 2011·Immunobiology·Blanca Estela García-PérezJulieta Luna-Herrera
Aug 5, 2014·PloS One·Shi-Yang TangKhashayar Khoshmanesh

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