LOX expression and functional analysis in astrocytomas and impact of IDH1 mutation

PloS One
Roseli da SilvaSueli M Oba-Shinjo

Abstract

Lysyl oxidase (LOX) is involved in vital biological processes such as cell motility, cell signaling and gene regulation. Deregulation of this protein can contribute to tumor formation and progression. Although it is known that LOX is involved in invasion, proliferation and tumor migration in other types of tumors, studies of LOX in astrocytomas of different grades are scarce. The purpose of our study was to characterize LOX, BMP1 and HIF1A expression by real-time PCR in astrocytomas with WHO grades I to IV compared to non-neoplastic brain tissue. IDH1 mutational status was determined by PCR and sequencing. LOX protein expression was also analyzed by immunohistochemistry. LOX functional analyses were performed using siRNA knockdown and the specific inhibitor BAPN in two glioblastoma cell lines. The expression levels of LOX, BMP1 and HIF1A were correlated and analyzed according to IDH1 mutation status and to the clinical end-point of overall survival of glioblastoma patients. The results demonstrate that increased expression and activity of LOX, BMP1 and HIF1A were positively correlated with the malignant grade of astrocytomas. LOX protein expression also increased according to the degree of malignancy, with localization in the c...Continue Reading

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Citations

Feb 11, 2016·The International Journal of Biochemistry & Cell Biology·Daming CuiLiang Gao
Dec 31, 2016·International Journal of Molecular Sciences·Tong-Hong WangTzong-Ming Shieh
Nov 23, 2017·Cells·Ana Rita MonteiroPatrícia A Madureira
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Jun 19, 2018·PloS One·Isabele Fattori MorettiSuely Kazue Nagahashi Marie
Jun 9, 2020·Cell Transplantation·Yunliang TangZhenguo Zeng
Aug 16, 2016·Cell and Tissue Research·Zareen Khan, John F Marshall
May 20, 2020·Cell Death and Differentiation·Hua YuXiongjun Wang
Feb 25, 2017·PloS One·Rahimsan K RamachandranBjarne W Kristensen
Apr 27, 2017·Biophysical Journal·Christopher WalterAmit Pathak

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Methods Mentioned

BETA
electrophoresis
PCR
PCRs
transfection

Software Mentioned

IDT
GraphPad Prism
SPSS
PICASA

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