PMID: 8947466Nov 15, 1996Paper

LP2, a differentiation-associated lipid-binding protein expressed in bovine lens

The Biochemical Journal
C Jaworski, G Wistow

Abstract

A 13 kDa protein from bovine lens was identified and characterized by protein microsequencing and by rapid amplification of cDNA ends (RACE) PCR. Its complete sequence shows that this protein belongs to a family of fatty acid-binding proteins (FABPs), including myelin and adipocyte P2, that are associated with cellular differentiation. The bovine lens protein, designated LP2, shows very close similarity to human epidermal FABP (eFABP) and human eFABP was detected in human lens, suggesting that the two proteins might be orthologous. Reverse transcriptase-PCR (RT-PCR) was used to compare expression patterns of LP2 with those for actin and for the differentiation markers gamma B-crystallin and gamma s-crystallin in lens. Actin was most abundant in the relatively undifferentiated epithelial cells and decreased with lens cell differentiation. In contrast gamma B-crystallin and gamma s-crystallin were detected only in fibres (nuclear and cortical respectively). LP2 transcripts were detected most abundantly in fibre cells and apparently increased with cellular differentiation. Molecular modelling confirms that the sequence of LP2 fits the tertiary template of adipocyte P2 but reveals the presence of two close pairs of cysteine residue...Continue Reading

Citations

Dec 31, 2000·The Journal of Biological Chemistry·D SinhaG Wistow
Jan 1, 1997·Annual Review of Nutrition·D A BernlohrL J Banaszak
Jan 10, 2006·Journal of Molecular Histology·Mohammad Reza NouraniHisatake Kondo
Apr 28, 2005·The FEBS Journal·Graeme WistowThomas Vihtelic
Aug 4, 2006·Journal of Medicinal Chemistry·Patricia A McDonnellBennett T Farmer
Jun 17, 2000·Trends in Endocrinology and Metabolism : TEM·A V Hertzel, D A Bernlohr
Jun 20, 1998·Biochimica Et Biophysica Acta·N R Coe, D A Bernlohr
Jun 3, 1999·Biochimica Et Biophysica Acta·R D ZinovievaS I Tomarev
Apr 14, 2000·Brain Research. Molecular Brain Research·G W AllenM De León
Mar 5, 2002·The Journal of Investigative Dermatology·Yuji OwadaHisatake Kondo
Oct 27, 2004·Experimental Eye Research·Martin IwigHans Gert Struck

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