PMID: 9541595Apr 29, 1998Paper

LPAM-1 (integrin alpha 4 beta 7)-ligand binding: overlapping binding sites recognizing VCAM-1, MAdCAM-1 and CS-1 are blocked by fibrinogen, a fibronectin-like polymer and RGD-like cyclic peptides

European Journal of Immunology
Y YangG W Krissansen

Abstract

The alpha 4 integrin LPAM-1 (alpha 4 beta 7) mediates lymphocyte attachment within the extracellular matrix (ECM) by adhering to the connecting segment (CS)-1 site of fibronectin (FN). Here we reveal that very late antigen (VLA)-4 LPAM-1+ T cell lymphoma TK-1 cells bind via LPAM-1 to multiple copies of the RGD sequence engineered within an FN-like polymer. Further, the small conformationally restrained RGD-like cyclic peptides 1-adamantaneacetyl-Cys-Gly-Arg-Gly-Asp-Ser-Pro-Cys and Arg-Cys-Asp-thioproline-Cys inhibit the adhesion of TK-1 cells to immobilized CS-1 peptide, and to endothelial counterreceptors for LPAM-1, namely mucosal addressin cell adhesion molecule (MAdCAM)-1 and vascular cell adhesion molecule (VCAM)-1. Spontaneous adhesion of the VLA-4- LPAM-1+ B lymphoma cell line RPMI 8866 to CS-1 was likewise inhibited, confirming a previously undocumented ability of LPAM-1 to recognize the RGD tripeptide. The RGD-binding site in LPAM-1 either overlaps or is identical to sites required for interaction with MAdCAM-1, VCAM-1, and the CS-1. The binding of LPAM-1 and VLA-4 to RGD-containing ligands may have relevance in vivo given that fibrinogen at physiological concentrations is able to partially block the binding of TK-1 ce...Continue Reading

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Citations

Feb 17, 2001·Infection and Immunity·J U IgietsemeL L Perry
Jul 18, 2009·Journal of Neurology·Azza IsmailBasil Sharrack
Jul 26, 2012·Neurological Research·Alireza MinagarJ Steven Alexander
Nov 24, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G M TanneauH P Salmon
May 8, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Eileen M FinneganDhananjay K Kaul
Sep 6, 2007·The Journal of Clinical Investigation·Eun Jeong ParkMotomu Shimaoka
Feb 22, 2017·Inflammatory Bowel Diseases·Sebastian ZundlerMarkus F Neurath
Jun 24, 1999·Cytokine & Growth Factor Reviews·A Meager
Mar 16, 2001·Progress in Neurobiology·S C PrevitaliH Hartung

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