LPP, a LIM protein highly expressed in smooth muscle

American Journal of Physiology. Cell Physiology
Isabelle GorenneAndrew P Somlyo

Abstract

An 80-kDa protein, prominently expressed in smooth muscle, was microsequenced and identified as LPP, the product of the lipoma-preferred partner gene (Petit MMR, Mols R, Schoenmakers EFPM, Mandahl N, and Van de Ven WJM. Genomics 36: 118-129, 1996). Using a specific anti-LPP antibody, we showed, in Western blots and with immunofluorescence microscopy, the selective expression of LPP in vascular and visceral smooth muscles (approximately 0.5-1 ng/microg total protein). In other mature (noncultured) tissues, including heart and skeletal muscle, the protein is present only in trace amounts and is closely correlated with the levels of the smooth muscle marker alpha-actin. In freshly isolated guinea pig bladder smooth muscle cells, immunofluorescence images showed LPP as linear arrays of punctate, longitudinally oriented staining superimposed with vinculin staining on the plasma membrane surface. A corresponding pattern of periodic labeling at the membrane in transverse sections of bladder smooth muscle suggested an association of LPP with peripheral dense bodies. In cultured rat aortic smooth muscle cells, LPP colocalized with vinculin at focal adhesions but not with p120 catenin or alpha-actinin. Overexpression of the protein incre...Continue Reading

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Citations

Jun 18, 2009·Physiological Genomics·Han ZhangCatherine S Chew
Jul 4, 2008·Circulation Research·Joseph M Miano
Jan 1, 2012·FEBS Open Bio·Charlotte L HooperSamuel Y Boateng
Aug 25, 2009·Translational Oncology·Thomas Gp GrunewaldElke Butt
Nov 30, 2004·Clinical and Experimental Pharmacology & Physiology·Darren J FernandesJulian Solway
Oct 14, 2017·Journal of Cell Communication and Signaling·Elaine NganPeter M Siegel
Aug 17, 2004·American Journal of Physiology. Heart and Circulatory Physiology·David Sanz-RosaVictoria Cachofeiro
Feb 18, 2006·Circulation Research·Mark W Majesky
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