LPS aggravates lung inflammation induced by RSV by promoting the ERK-MMP-12 signaling pathway in mice.

Respiratory Research
Shenglin ChenEnmei Liu

Abstract

RSV can lead to persistent airway inflammation and airway hyperresponsiveness (AHR), and is intimately associated with childhood recurrent wheezing and asthma, but the underlying mechanisms remain unclear. Lipopolysaccharide (LPS) is also implicated in the onset and exacerbation of asthma. However, whether inhalation of LPS can boost airway inflammation induced by RSV is not clear. In this study, we utilized an LPS- and RSV-superinfected mouse model to explore underlying pathogenesis. Mice were infected with RSV on day 0 and inoculated with LPS from day 35 to day 41, samples were collected on day 42. Inflammatory cells, lung histopathology and AHR were measured. Cytokines were detected by ELISA and ERK, JNK, p38 was determined by western blot. MMP408, PD98059, SP600125 and SB203580 were used to inhibit MMP-12, ERK, JNK and p38 respectively. LPS exposure superimposed on RSV-infected lungs could lead to more vigorous cellular influx, lung structures damage, augmented AHR and higher MMP-12 levels. Inhibition of MMP-12 or ERK signaling pathway in vivo both diminished LPS-driven airway inflammation and AHR. Exposure to LPS in RSV-infected mice is associated with enhanced increases in ERK-MMP-12 expression that translates into increa...Continue Reading

References

Apr 27, 2000·American Journal of Respiratory Cell and Molecular Biology·M K TulićP D Sly
Sep 20, 2002·The New England Journal of Medicine·Charlotte Braun-FahrländerUNKNOWN Allergy and Endotoxin Study Team
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dunia RodríguezMomtchilo Russo
Jun 30, 2006·Journal of the Air & Waste Management Association·C Arden Pope, Douglas W Dockery
Feb 29, 2008·Cytokine·Yong-Chen LuPamela S Ohashi
Feb 7, 2012·The Journal of Allergy and Clinical Immunology·Rekha ChaudhuriNeil C Thomson
Mar 2, 2013·American Journal of Respiratory and Critical Care Medicine·F Martín FerollaFernando P Polack
May 3, 2014·Nature Medicine·David J MarchantChristopher M Overall
Jan 3, 2015·The Journal of Clinical Investigation·Mauricio T CaballeroFernando P Polack
Nov 14, 2015·Tissue Engineering. Part a·Stephen Lee RegoKhalil N Bitar
Mar 19, 2016·International Journal of Occupational Medicine and Environmental Health·Nora KovátsCsaba Fekete
Sep 2, 2016·American Journal of Respiratory and Critical Care Medicine·Cristine E BerryFernando D Martinez
Sep 3, 2016·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Shengtao ZhaoChangzheng Wang
Feb 6, 2017·Molecular Neurobiology·Bharath ChelluboinaKrishna Kumar Veeravalli
Jul 1, 2017·Progress in Molecular Biology and Translational Science·Amanda Y Hendrix, Farrah Kheradmand
Jan 10, 2018·American Journal of Respiratory Cell and Molecular Biology·Yohannes A Mebratu, Yohannes Tesfaigzi
Sep 28, 2018·International Forum of Allergy & Rhinology·Neil N PatelNoam A Cohen

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Methods Mentioned

BETA
bronchoalveolar lavage
FACS
enzyme-linked immunosorbent assay
PCR
flow cytometry
ELISA

Software Mentioned

ImageJ2x
Prism GraphPad

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