PMID: 2485079Jan 1, 1989Paper

LPS augments adoptive transfer of experimental allergic encephalomyelitis in the Lewis rat

Autoimmunity
T HamadaE C Alvord

Abstract

Adoptive transfer of experimental allergic encephalomyelitis (EAE) is enhanced after in vitro culture of myelin basic protein (BP)-sensitized lymphoid cells with BP. Addition of lipopolysaccharide (LPS) to the culture further augments transfer of EAE to a level 5 times greater than that achieved with cells activated only with BP. Neither the proliferative response of a BP-specific cell line nor the production of IL-2 by BP-sensitized lymphoid cells in response to BP was augmented by the addition of LPS to the culture. Augmentation of EAE was also observed if recipients received simultaneous injections of BP-sensitized lymph node cells (BP/LNC) cultured with BP (BP-activated) and normal spleen cells cultured independently with LPS (LPS/Spl-C). To analyze the effect of contact between these two cell populations in vivo, we mixed the two cell populations in vitro at reduced cell concentrations. When BP-activated BP-LNC were mixed with LPS-Spl-C in vitro, a marked synergistic proliferative response was observed. Irradiation of BP-activated BP/LNC abrogated this synergistic response, whereas irradiation of LPS/Spl-C did not, suggesting that the proliferating population was in the BP/LNC and that the LPS/Spl-C enhanced their prolifer...Continue Reading

Citations

Nov 3, 2010·Expert Opinion on Biological Therapy·Felix I L Clanchy, Sandra M Sacre
Jul 11, 2006·Journal of Neuroimmunology·Jason R LeesJohn H Russell
Aug 17, 2005·Scandinavian Journal of Immunology·S YoshinoH Takano
Sep 29, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·G Cavicchioni, S Spisani
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Axel NogaiThomas Kamradt
Jan 7, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lizette VisserJon D Laman

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