LPS-mediated cell surface expression of CD74 promotes the proliferation of B cells in response to MIF

Cellular Signalling
Christina KlasenOmar El Bounkari

Abstract

Macrophage migration inhibitory factor (MIF) is a chemokine-like inflammatory cytokine, which plays a pivotal role in the pathogenesis of inflammatory and cardiovascular diseases as well as cancer. We previously identified MIF as a novel B cell chemokine that promotes B cell migration through non-cognate interaction with the CXC chemokine receptor CXCR4 and CD74, the surface form of MHC class II invariant chain. In this study, we have analyzed the regulation of the MIF receptors under inflammatory conditions by investigating the impact of lipopolysaccharide (LPS), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) on CD74 and CXCR4 expression in B lymphocytes. We found that both LPS and TNF-α stimulation of primary B cells and the human B myeloma cell line RPMI-8226 enhanced protein expression as well as mRNA levels of CD74 in a time- and dose-dependent manner. By contrast, no effect on CXCR4 expression was observed. Selective inhibition of IκBα phosphorylation significantly attenuated LPS-induced expression of CD74, suggesting the contribution of NF-κB signaling pathways to the regulation of CD74 expression. Importantly, individual or simultaneous blockade of MIF or CD74 using specific neutralizing antibodies markedly ...Continue Reading

Citations

Feb 10, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mohammed HowaitAlexandru Movila
Mar 27, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Laura Florez-SampedroBarbro N Melgert
Nov 27, 2018·Biosensors & Bioelectronics·Ji-Hong BongJae-Chul Pyun
Aug 11, 2019·Cytokine·Stanislava Stošić-GrujičićIvana Stojanović
Apr 6, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hong-Xia WangYu-Rong Qiu
Jul 14, 2021·EMBO Molecular Medicine·Mirco FriedrichLukas Bunse
Aug 25, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jaewon J LeePaola A Guerrero

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