PMID: 8955407Dec 1, 1996Paper

Lrp is a direct repressor of the dad operon in Escherichia coli

Journal of Bacteriology
E MathewM Freundlich

Abstract

Expression of the degradative D-amino acid dehydrogenase (dad) operon is known to be increased when Escherichia coli is grown in the presence of D- or L-alanine. Alanine is thought to act as an inducer to block the action of a postulated repressor. This operon is also believed to be regulated by catabolite repression. We have used in vivo and in vitro experiments that show that the dad repressor is the leucine-responsive regulatory protein (Lrp). dad expression in a dad-lacZ operon fusion strain was increased four- to sevenfold when cells were grown in minimal medium containing alanine or leucine. A strain lacking Lrp had high-level constitutive dad expression. Gel retardation and footprinting studies revealed that Lrp binds in vitro to multiple sites over a large area in the dad promoter region. This binding was reduced by alanine or leucine. In vitro transcription assays, using a plasmid template and primer extension analysis, identified three major dad transcripts (Tr1, Tr2, and Tr3). The formation of these transcripts was differentially regulated by cyclic AMP-cyclic AMP receptor protein complex, and each was strongly repressed by Lrp. Alanine or leucine completely (for Tr1 and Tr2) or partially (for Tr3) reversed Lrp inhib...Continue Reading

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Citations

Apr 5, 2003·Molecular Microbiology·Arie B BrinkmanJohn van der Oost
Jun 4, 2002·The Journal of Biological Chemistry·Arie B BrinkmanJohn van der Oost
May 4, 2016·Nucleic Acids Research·Hyun-Jung Lee, Susan Gottesman
Dec 21, 2010·Journal of Bacteriology·Benjamin R Hart, Robert M Blumenthal
Sep 7, 2000·The Journal of Biological Chemistry·A B BrinkmanJ van Der Oost
Dec 26, 2006·Nucleic Acids Research·Ryan K ShultzabergerThomas D Schneider
Feb 13, 2007·Journal of Bacteriology·Sergio BodiniFrancesca Santori
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Jan 11, 2005·Journal of Biotechnology·T PolenV F Wendisch

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