Lrp5 is not required for the proliferative response of osteoblasts to strain but regulates proliferation and apoptosis in a cell autonomous manner.

PloS One
Behzad JavaheriJoanna S Price

Abstract

Although Lrp5 is known to be an important contributor to the mechanisms regulating bone mass, its precise role remains unclear. The aim of this study was to establish whether mutations in Lrp5 are associated with differences in the growth and/or apoptosis of osteoblast-like cells and their proliferative response to mechanical strain in vitro. Primary osteoblast-like cells were derived from cortical bone of adult mice lacking functional Lrp5 (Lrp5(-/-)), those heterozygous for the human G171V High Bone Mass (HBM) mutation (LRP5(G171V)) and their WT littermates (WT(Lrp5), WT(HBM)). Osteoblast proliferation over time was significantly higher in cultures of cells from LRP5(G171V) mice compared to their WT(HBM) littermates, and lower in Lrp5(-/-) cells. Cells from female LRP5(G171V) mice grew more rapidly than those from males, whereas cells from female Lrp5(-/-) mice grew more slowly than those from males. Apoptosis induced by serum withdrawal was significantly higher in cultures from Lrp5(-/-) mice than in those from WT(HBM) or LRP5(G171V) mice. Exposure to a single short period of dynamic mechanical strain was associated with a significant increase in cell number but this response was unaffected by genotype which also did not cha...Continue Reading

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Citations

Mar 20, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lee B MeakinJoanna S Price
Aug 10, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Behzad JavaheriMark L Johnson
Jun 7, 2015·Reviews in Endocrine & Metabolic Disorders·N Lara-Castillo, M L Johnson
Sep 4, 2015·BoneKEy Reports·Robin Michael Delaine-SmithRobin Mark Howard Rumney
Jan 14, 2017·Cell Biochemistry and Function·Rosemary F L SuswilloAndrew A Pitsillides
Jul 30, 2014·The Journal of Biological Chemistry·Gabriel L GaleaJoanna S Price
Nov 25, 2019·Current Osteoporosis Reports·Behzad Javaheri, Andrew A Pitsillides

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Methods Mentioned

BETA
transgenic
chip
FCS

Software Mentioned

SAS
SPSS
Leica Qwin
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis