LRP5 is required for vascular development in deeper layers of the retina.

PloS One
Chun-hong XiaXiaohua Gong

Abstract

The low-density lipoprotein receptor-related protein 5 (LRP5) plays an important role in the development of retinal vasculature. LRP5 loss-of-function mutations cause incomplete development of retinal vessel network in humans as well as in mice. To understand the underlying mechanism for how LRP5 mutations lead to retinal vascular abnormalities, we have determined the retinal cell types that express LRP5 and investigated specific molecular and cellular functions that may be regulated by LRP5 signaling in the retina. We characterized the development of retinal vasculature in LRP5 mutant mice using specific retinal cell makers and a GFP transgene expressed in retinal endothelial cells. Our data revealed that retinal vascular endothelial cells predominantly formed cell clusters in the inner-plexiform layer of LRP5 mutant retina rather than sprouting out or migrating into deeper layers to form normal vascular network in the retina. The IRES-beta-galactosidase (LacZ) report gene under the control of the endogenous LRP5 promoter was highly expressed in Müller cells and was also weakly detected in endothelial cells of the retinal surface vasculature. Moreover, the LRP5 mutant mice had a reduction of a Müller cell-specific glutamine tr...Continue Reading

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Citations

Aug 9, 2013·Molecular Neurobiology·Sung Hoon LeeChan Young Shin
Jan 8, 2014·Documenta Ophthalmologica. Advances in Ophthalmology·Machelle T Pardue, Neal S Peachey
Jun 23, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Pascal StuelsatzZipora Yablonka-Reuveni
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Oct 20, 2018·Journal of Cell Science·Catherine ChengVelia M Fowler
Jul 26, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chi-Hsiu LiuJing Chen
May 31, 2018·Investigative Ophthalmology & Visual Science·Shruti SharmaAshok Sharma
May 3, 2020·Development·Saptarshi BiswasDritan Agalliu
Apr 4, 2021·Pharmaceuticals·Tyler SniegowskiVadivel Ganapathy

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Methods Mentioned

BETA
reverse transcription-PCR
PCR
genotyping
FCS
electrophoresis

Software Mentioned

Diagnosys
Espion

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