Apr 30, 2020

LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells

Stem Cell Research & Therapy
Fei HeFu-Rong Li


Human embryonic stem cells represent a potentially unlimited source of insulin-producing cells for diabetes therapy. While tremendous progress has been made in directed differentiation of human embryonic stem cells into IPCs in vitro, the mechanisms controlling its differentiation and function are not fully understood. Previous studies revealed that lysine-specific demethylase 1(LSD1) balanced the self-renewal and differentiation in human induced pluripotent stem cells and human embryonic stem cells. This study aims to explore the role of LSD1 in directed differentiation of human embryonic stem cells into insulin-producing cells. Human embryonic stem cell line H9 was induced into insulin-producing cells by a four-step differentiation protocol. Lentivirus transfection was applied to knockdown LSD1 expression. Immunofluorescence assay and flow cytometry were utilized to check differentiation efficiency. Western blot was used to examine signaling pathway proteins and differentiation-associated proteins. Insulin/C-peptide release was assayed by ELISA. Statistical analysis between groups was carried out with one-way ANOVA tests or a student's t test when appropriate. Inhibition or silencing LSD1 promotes the specification of pancrea...Continue Reading

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Mentioned in this Paper

Diabetes Therapy
ERK Signal Tranduction Pathway
NKX6-1 gene
Human Embryonic Stem Cells
Genus: Lentivirus group
Enzyme-Linked Immunosorbent Assay
TADG-12 serine protease
Insulin-binding peptide

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