lsm1 mutations impairing the ability of the Lsm1p-7p-Pat1p complex to preferentially bind to oligoadenylated RNA affect mRNA decay in vivo.

RNA
Ashis Chowdhury, S Tharun

Abstract

The poly(A) tail is a crucial determinant in the control of both mRNA translation and decay. Poly(A) tail length dictates the triggering of the degradation of the message body in the major 5' to 3' and 3' to 5' mRNA decay pathways of eukaryotes. In the 5' to 3' pathway oligoadenylated but not polyadenylated mRNAs are selectively decapped in vivo, allowing their subsequent degradation by 5' to 3' exonucleolysis. The conserved Lsm1p-7p-Pat1p complex is required for normal rates of decapping in vivo, and the purified complex exhibits strong binding preference for oligoadenylated RNAs over polyadenylated or unadenylated RNAs in vitro. In the present study, we show that two lsm1 mutants produce mutant complexes that fail to exhibit such higher affinity for oligoadenylated RNA in vitro. Interestingly, these mutant complexes are normal with regard to their integrity and retain the characteristic RNA binding properties of the wild-type complex, namely, binding near the 3'-end of the RNA, having higher affinity for unadenylated RNAs that carry U-tracts near the 3'-end over those that do not and exhibiting similar affinities for unadenylated and polyadenylated RNAs. Yet, these lsm1 mutants exhibit a strong mRNA decay defect in vivo. Thes...Continue Reading

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Citations

Apr 21, 2010·The Journal of Cell Biology·Gabrielle HaasElisa Izaurralde
Jun 15, 2010·The EMBO Journal·Joerg E BraunElisa Izaurralde
Feb 27, 2016·RNA Biology·Mridula MuppavarapuTracy Nissan
Jan 24, 2012·Biochimica Et Biophysica Acta·Vidya BalagopalTracy Nissan
Feb 9, 2013·RNA Biology·Carol J Wilusz, Jeffrey Wilusz
Jul 21, 2016·PloS One·Ashis ChowdhurySundaresan Tharun
Dec 2, 2010·Biochemical Society Transactions·Aline Marnef, Nancy Standart
Aug 25, 2010·The Journal of Biological Chemistry·Tatsuhisa Tsuboi, Toshifumi Inada
Jul 19, 2014·RNA·Ashis ChowdhurySundaresan Tharun

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