LTR sequence of the MDG4 retrotransposon contains the MAD protein binding site that affects the east-dependent repression

Doklady. Biochemistry and Biophysics
Anton GolovninLarisa Melnikova

Abstract

Earlier, we showed in a model system of the yellow gene of D. melanogaster that an increase in the EAST protein concentration leads to repression in bristles, the mechanism of which remained obscure. In this study, an inverted repeat was localized by genetic methods in the long terminal repeat (LTR) sequence of the MDG4 retrotransposon. This repeat includes the binding site for the Mad protein-the key component of the of TGF-β/BMP signaling cascade. The results of this work suggest that the Mad protein recruits to chromatin a regulatory complex that functionally interacts with the EAST protein. This complex either itself suppresses the yellow gene expression in bristles or moves the transgene to the nuclear regions with a high concentration of transcription repression factors.

References

May 12, 2000·Nature Cell Biology·M Wasser, W Chia
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·J Massagué
Dec 3, 2004·Molecular Cell·Chi-Yun PaiVictor G Corces
Oct 13, 2007·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·L S Mel'nikovaA K Golovnin

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