Luciferase from Fulgeochlizus bruchi (Coleoptera:Elateridae), a Brazilian click-beetle with a single abdominal lantern: molecular evolution, biological function and comparison with other click-beetle luciferases

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
D T AmaralV R Viviani

Abstract

Bioluminescent click-beetles emit a wide range of bioluminescence colors (λ(Max) = 534-594 nm) from thoracic and abdominal lanterns, which are used for courtship. Only the luciferases from Pyrophorus and Pyrearinus species were cloned and sequenced. The Brazilian Fulgeochlizus bruchi click-beetle, which inhabits the Central-west Cerrado (Savannas), is noteworthy because, differently from other click-beetles, the adult stage displays only a functional abdominal lantern, which produces a bright green bioluminescence for sexual attraction purposes, and lacks functional thoracic lanterns. We cloned the cDNA for the abdominal lantern luciferase of this species. Notably, the primary sequence of this luciferase showed slightly higher identity with the green emitting dorsal lantern luciferases of the Pyrophorus genus instead of the abdominal lanterns luciferases. This luciferase displays a blue-shifted spectrum (λ(Max) = 540 nm), which is pH-insensitive from pH 7.5 to 9.5 and undergoes a slight red shift and broadening above this pH; the lowest K(M) for luciferin among studied click-beetle luciferases, and the highest optimum pH (9.0) ever reported for a beetle luciferase. At pH 9.0, the K(M) for luciferin increases, showing a decrease...Continue Reading

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Citations

Jul 28, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Danilo T AmaralVadim R Viviani
Nov 3, 2017·Luminescence : the Journal of Biological and Chemical Luminescence·Gabriela Oliveira, Vadim R Viviani
Jul 23, 2013·Luminescence : the Journal of Biological and Chemical Luminescence·D T AmaralV R Viviani
May 21, 2015·Photochemistry and Photobiology·Jamileh GharanlarParichehr Yaghmaei
May 21, 2013·Photochemistry and Photobiology·Yasuo MitaniYoshihiro Ohmiya
Nov 5, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·V R VivianiV R Bevilaqua
May 13, 2020·Journal of Chemical Theory and Computation·Mohan Yu, Ya-Jun Liu

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