PMID: 9635135Jun 23, 1998Paper

Lucigenin reduction by NADPH-cytochrome P450 reductase and the effect of phospholipids and albumin on chemiluminescence

Biochemistry and Molecular Biology International
M TsukamotoM Yonaha

Abstract

To assess lucigenin, a chemilumigenic probe, as a detector of superoxide anion in microsomes, NADPH oxidation, lucigenin disappearance, and chemiluminescence in a system including purified NADPH-cytochrome P450 reductase were examined. NADPH oxidation was increased by adding lucigenin, and concurrently, its disappearance and oxygen consumption were also stimulated. Chemiluminescence, which is negligibly emitted in the presence of the reductase alone, was remarkably amplified with phospholipids and albumin. Menadione inhibited lucigenin disappearance resulting in suppression of chemiluminescence. Lucigenin chemiluminescence measured in microsomes appears not to reflect direct superoxide anion production from microsomal components and from quinones, such as menadione.

Citations

Aug 10, 2005·Free Radical Research·Alexandra KrauskopfTimo M Buetler
Jul 22, 2019·Nature Communications·Grazia CamardaGiancarlo A Biagini

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