PMID: 11334624May 4, 2001Paper

Lumen geometry of ion channels formed by Vibrio cholerae EL Tor cytolysin elucidated by nonelectrolyte exclusion

Biochimica Et Biophysica Acta
Liliya N YuldashevaOleg V Krasilnikov

Abstract

Vibrio cholerae EL Tor cytolysin, a water-soluble protein with a molecular mass of 63 kDa, forms small pores in target cell membranes. In this communication, planar lipid bilayers under voltage clamp conditions were used to investigate the geometric properties of the pores. It was established that all cytolysin channels were inserted into membranes with the same orientation. Sharp asymmetry in the I-V curve of fully open cytolysin channels persisting at high electrolyte concentrations indicated asymmetry in the geometry of the channel lumen. Using the nonelectrolyte exclusion method, evidence was obtained that the cis opening of the channel had a larger diameter (< or = 1.9 nm) than the trans opening (< or = 1.6 nm). The channel lumen appeared constricted, with a diameter of < or = 1.2 nm. Cup-shaped lumen geometry was deduced for both channel openings, which appeared to be connected to each other via a central narrow part. The latter contributed significantly to the total electrical resistance and determined the discontinuous character of channel filling with nonelectrolytes. Comparisons of the properties of pores formed by cytolysins of two V. cholerae biotypes (EL Tor and non-O1) indicated that the two ion channels possessed...Continue Reading

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Citations

Feb 6, 2004·Toxicon : Official Journal of the International Society on Toxinology·Karina N LaohachaiJoseph I Kourie
Oct 13, 2012·Chemical Reviews·Ekaterina M Nestorovich, Sergey M Bezrukov
Nov 10, 2009·Journal of Bacteriology·Takashige KashimotoNobuyuki Susa
Feb 10, 2007·FEBS Letters·Olga S OstroumovaSergey M Bezrukov
Oct 4, 2015·Biochimica Et Biophysica Acta·John J KasianowiczJoseph W F Robertson
Dec 19, 2002·Biochimica Et Biophysica Acta·Olivier PeyronnetJean-Louis Schwartz
Dec 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Sergio Pantano, Cesare Montecucco
Jun 6, 2003·Biochimica Et Biophysica Acta·Carlos M M CarneiroOleg V Krasilnikov
Aug 5, 2009·Biophysical Journal·Ronald SoongPeter M Macdonald
Aug 26, 2014·Toxins·Philip A Gurnev, Ekaterina M Nestorovich

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