PMID: 8944559Oct 1, 1996Paper

Luminal bacteria and proteases together decrease adherence of Entamoeba histolytica trophozoites to Chinese hamster ovary epithelial cells: a novel host defence against an enteric pathogen

Gut
Easwaran P Variyam

Abstract

Factors that prevent colonic mucosal invasion by pathogenic Entamoeba histolytica are not understood. A key initial step in pathogenesis of injury induced by amoeba is adherence to target cells mediated by a surface glycoprotein lectin on E histolytica. Mucin degrading bacteria normally present in the colon lumen produce glycosidases that degrade soluble or cell surface glycoconjugates. To determine whether glycosidases produced by mucin degrading bacteria, alone or in combination with proteases present in colon lumen, can decrease E histolytica adherence to target epithelial cells by degrading E histolytica adherence lectin. The effects of exposure of E histolytica trophozoites strains HM1:IMSS and 200:NIH to faecal culture supernatant fluids, culture supernatant preparations of mucin degrading bacteria, and luminal proteases on their adherence to Chinese hamster ovary (CHO) cells were determined. The amount of surface adherence lectin on E histolytica trophozoites before and after treatment with glycosidases and proteases was determined by immunofluorescence. The effect of glycosidases and proteases on purified E histolytica lectin was determined by gel electrophoresis. Incubation of E histolytica with culture supernatant pre...Continue Reading

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Citations

Nov 7, 2000·Parasitology Today·S J HicksA P Corfield
Nov 9, 2000·Archives of Medical Research·E P VariyamK Eaton
Apr 21, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Alfonso Olivos-GarcíaRuy Pérez-Tamayo
Dec 17, 2008·International Journal for Parasitology·Espiridión Ramos-MartínezRuy Pérez-Tamayo
Aug 19, 2007·Current Opinion in Immunology·Xiaoti GuoWilliam A Petri
Jan 30, 2007·Trends in Parasitology·Easwaran P Variyam
Mar 15, 2000·Journal of Molecular Biology·S Moody-HauptM J McConville

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