Luminal endocytosis and intracellular targeting by acinar cells during early biliary pancreatitis in the opossum

The Journal of Clinical Investigation
M M LerchM L Steer

Abstract

Cell necrosis in acute experimental pancreatitis is preceded by a redistribution of digestive enzymes into a lysosomal subcellular compartment. We have investigated whether endocytosis from the acinar cell lumen might contribute to this disturbance of intracellular compartmentation. In an animal model of pancreatitis involving pancreatic bile duct ligation in opossums, we have studied in vivo endocytosis of dextran 40 and [14C]dextran 70, cationized ferritin, and horseradish peroxidase from the apical surface of acinar cells before the onset of necrosis. Marker solutions were instilled into the pancreatic duct of anesthetized animals at physiological pressure. Tissue samples obtained at intervals of up to 60 min after instillation of markers were studied by electron microscopy and electron microscope autoradiography. All markers were taken up by acinar cells in control animals and in animals with obstructed pancreatic bile ducts. Markers for membrane-mediated endocytosis (cationated ferritin and horseradish peroxidase) were transported to lysosomes in both groups. In contrast, the fluid-phase tracer dextran was transported to the secretory pathway in controls but to lysosomes after duct ligation. Since dextran and luminally pre...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·V Herzog, M G Farquhar
Feb 1, 1991·Scandinavian Journal of Gastroenterology·R BialekG Adler
Jan 1, 1991·Journal of Electron Microscopy Technique·A R Beaudoin, G Grondin
Oct 1, 1989·The Journal of Clinical Investigation·A SalujaM Steer
Jan 1, 1989·Pancreas·H YamaguchiH Nawata
Feb 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Livne, C Oliver
May 1, 1972·The Journal of Cell Biology·N SimionescuG E Palade
Feb 1, 1973·The New England Journal of Medicine·R Blum, S K Carter
Feb 28, 1974·The New England Journal of Medicine·J M Acosta, C L Ledesma
Jul 1, 1974·The American Journal of Digestive Diseases·M C Geokas, H Rinderknecht
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
Oct 6, 1994·The New England Journal of Medicine·M M LerchG Adler
May 29, 1993·Lancet·C A Hernández, M M Lerch

❮ Previous
Next ❯

Citations

May 5, 2005·Experimental Cell Research·Robert C De Lisle
Mar 17, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mark W SherwoodAlexei V Tepikin
Jul 23, 1999·Annals of the New York Academy of Sciences·J SchnekenburgerM M Lerch
Oct 25, 2006·Annual Review of Physiology·Ashok K SalujaVikas Dudeja
Oct 6, 2010·The Journal of Clinical Investigation·Jochen ReiserThomas Reinheckel
Sep 21, 2000·The Journal of Clinical Investigation·W HalangkJ Deussing
Dec 3, 2011·Fibrogenesis & Tissue Repair·Alexander A AghdassiMarkus M Lerch
May 1, 2007·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·L P van MinnenL M A Akkermans
Apr 30, 2013·Gastroenterology·Markus M Lerch, Fred S Gorelick
Dec 26, 2009·Gastroenterology·Markus M Lerch, Ali A Aghdassi
Jul 16, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xianbao ZhanBaoan Ji
Aug 4, 1998·The American Journal of Physiology·B HofbauerM L Steer
Aug 25, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Svetlana G VoroninaAlexei V Tepikin
Sep 9, 1998·The American Journal of Physiology·D R GiovannucciE L Stuenkel
Aug 1, 2019·Cellular and Molecular Life Sciences : CMLS·Sudarshan R MallaJulia Mayerle
Jan 11, 2003·The Journal of Biological Chemistry·Frank Ch MoorenMarkus M Lerch
Jan 9, 2020·Gastroenterology·Matthias Sendler, Markus M Lerch
Jul 22, 2020·Gastroenterology·Markus M LerchMatthias Sendler
Jan 21, 2019·Gastroenterology·Julia MayerleMiklós Sahin-Tóth
Apr 28, 2018·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Markus M Lerch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.