PMID: 8969468Jun 1, 1996Paper

Luminal proteinases from Plodia interpunctella and the hydrolysis of Bacillus thuringiensis CryIA(c) protoxin

Insect Biochemistry and Molecular Biology
B OppertW H McGaughey

Abstract

The ability of proteinases in gut extracts of the Indianmeal moth, Plodia interpunctella, to hydrolyze Bacillus thuringiensis (Bt) protoxin, casein, and rho-nitroanilide substrates was investigated. A polyclonal antiserum to protoxin CryIA(c) was used in Western blots to demonstrate slower protoxin processing by gut enzymes from Bt subspecies entomocidus-resistant larvae than enzymes from susceptible or kurstaki-resistant strains. Enzymes from all three strains hydrolyzed N-alpha-benzoyl-L-arginine rho-nitroanilide, N-succinyl-ala-ala-pro-phenylalanine rho-nitroanilide, and N-succinyl-ala-ala-pro-leucine rho-nitroanilide. Zymograms and activity blots were used to estimate the apparent molecular masses, number of enzymes, and relative activities in each strain. Several serine proteinase inhibitors reduced gut enzyme activities, with two soybean trypsin inhibitors, two potato inhibitors, and chymostatin the most effective in preventing protoxin hydrolysis.

References

Nov 1, 1979·Analytical Biochemistry·E G DelMarM C Geokas
Feb 5, 1980·Biochemistry·E G Del MarM C Geokas

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Citations

Aug 31, 1999·Archives of Insect Biochemistry and Physiology·B Oppert
Sep 25, 2007·Biotechnology Letters·Madhusudhan BudathaAparna Dutta-Gupta
May 7, 1998·Insect Biochemistry and Molecular Biology·Y C ZhuA K Dowdy
Aug 23, 2002·Bulletin of Entomological Research·B OppertM Zuercher
Mar 7, 2001·Applied and Environmental Microbiology·S HerreroJ Ferré
Nov 5, 2005·Applied and Environmental Microbiology·Ali H SayyedNeil Crickmore
Dec 1, 2001·Annual Review of Entomology·Juan Ferré, Jeroen Van Rie
Feb 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·M Mahbubur RahmanOtto Schmidt
May 30, 2012·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Brenda OppertElena N Elpidina
Jan 29, 2008·Toxicon : Official Journal of the International Society on Toxinology·Lianet Rodríguez-CabreraCamilo Ayra-Pardo
Dec 6, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Lohitash KarumbaiahMichael J Adang
Nov 30, 2012·Archives of Insect Biochemistry and Physiology·Guillaume TetreauLaurence Després
Sep 8, 2004·Biochemical and Biophysical Research Communications·Huarong LiFangneng Huang
Jul 21, 2016·Current Opinion in Insect Science·Anaïs Castagnola, Juan Luis Jurat-Fuentes
Jul 16, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Renaud StalinskiLaurence Després
Oct 30, 2016·Toxicon : Official Journal of the International Society on Toxinology·Patricia Hernández-MartínezBaltasar Escriche
Mar 24, 2017·Journal of Economic Entomology·B PetersonJ Van den Berg
Feb 26, 2003·Molecular & Cellular Proteomics : MCP·Mehmet CandasLee A Bulla
Sep 20, 1997·The Journal of Biological Chemistry·B OppertW H McGaughey

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