Luminescence based enzyme-labeled phage (Phazyme) assays for rapid detection of Shiga toxin producing Escherichia coli serogroups.

Bacteriophage
John D WillfordLawrence D Goodridge

Abstract

Most diagnostic approaches for Shiga toxin producing Escherichia coli (STEC) have been designed to detect only serogroup O157 that causes a majority, but not all STEC related outbreaks in the United States. Therefore, there is a need to develop methodology that would enable the detection of other STEC serogroups that cause disease. Three bacteriophages (phages) that infect STEC serogroups O26, O103, O111, O145 and O157 were chemically labeled with horseradish peroxidase (HRP). The enzyme-labeled phages (Phazymes) were individually combined with a sampling device (a swab), STEC serogroup-specific immunomagnetic separation (IMS) beads, bacterial enrichment broth and luminescent HRP substrate, in a self-contained test device, while luminescence was measured in a hand-held luminometer.The O26 and O157 Phazyme assays correctly identified more than 93% of the bacteria tested during this study, the O123 Phazyme assay identified 89.6%, while the O111 and O145 Phazyme assays correctly detected 82.4% and 75.9%, respectively. The decreased specificity of the O111 and O145 assays was related to the broad host ranges of the phages used in both assays. The Phazyme assays were capable of directly detecting between 10(5) and 10(6) CFU/ml in pu...Continue Reading

References

Aug 1, 1992·Journal of Clinical Microbiology·A CordovézM M Levine
Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·T J WalshP Martino
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Jun 10, 1998·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·A HermanZ Weinraub
Aug 10, 2000·Molecular Biotechnology·E Schenborn, D Groskreutz
Feb 15, 2001·AIDS Care·R ColebundersUNKNOWN Eurosupport Group
Nov 13, 2003·Applied Spectroscopy·P A Mosier-BossM Breitbart
Jan 9, 2004·Applied and Environmental Microbiology·Masahito OdaYasunori Tanji
Oct 7, 2004·Journal of Biotechnology·Yasunori TanjiHajime Unno
Dec 21, 2004·Journal of Biotechnology·Masatoshi YoichiYasunori Tanji
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rotem EdgarSankar Adhya
Nov 7, 2006·Journal of Animal Science·C L Gyles
Jan 5, 2011·Emerging Infectious Diseases·Elaine ScallanPatricia M Griffin

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Citations

Jun 13, 2013·Foodborne Pathogens and Disease·Fei WangBeilei Ge
Feb 18, 2014·Bacteriophage·Mathias Schmelcher, Martin J Loessner
Mar 25, 2014·The Analyst·R G van der MerweN C Gey van Pittius
Oct 16, 2012·Virology·Marine Henry, Laurent Debarbieux
Jan 12, 2019·Annual Review of Food Science and Technology·Lisa O'SullivanAidan Coffey

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