Luminescence, circular dichroism and in silico studies of binding interaction of synthesized naphthylchalcone derivatives with bovine serum albumin

Luminescence : the Journal of Biological and Chemical Luminescence
Sharda PasrichaShefali Shukla

Abstract

Chalcones possess various biological properties, for example, antimicrobial, anti-inflammatory, analgesic, antimalarial, anticancer, antiprotozoal and antitubercular activity. In this study, naphthylchalcone derivatives were synthesized and characterized using 1 H NMR 13 C NMR, Fourier transform infrared and mass techniques. Yields for all derivatives were found to be >90%. Protein-drug interactions influence the absorption, distribution, metabolism and excretion (ADME) properties of a drug. Therefore, to establish whether the synthesized naphthylchalcone derivatives can be used as drugs, their binding interaction toward a serum protein (bovine serum albumin) was investigated using fluorescence, circular dichroism and molecular docking techniques under physiological conditions. Fluorescence quenching of the protein in the presence of naphthylchalcone derivatives, and other derived parameters such as association constants, number of binding sites and static quenching involving confirmed non-covalent binding interactions in the protein-ligand complex were observed. Circular dichroism clearly showed changes in the secondary structure of the protein in the presence of naphthylchalcones, indicating binding between the derivatives an...Continue Reading

References

Jan 1, 1985·Advances in Protein Chemistry·T Peters
Mar 29, 2001·Journal of Pharmacological and Toxicological Methods·C A Lipinski
Mar 19, 2004·Journal of Medicinal Chemistry·Thomas A HalgrenJay L Banks
Sep 2, 2004·Chemical & Pharmaceutical Bulletin·Jaldappa Seetharamappa, Bhalchandra Purushottam Kamat
Mar 14, 2007·Current Drug Targets·José H PereiraDiógenes S Santos
Apr 28, 2007·Physical Chemistry Chemical Physics : PCCP·Benjamin M BulhellerJonathan D Hirst
Aug 23, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·N WangJ X Yu
May 8, 2009·Chemical & Pharmaceutical Bulletin·Himanshu OjhaNabo Kumar Chaudhury
Jul 27, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aurica Varlan, Mihaela Hillebrand
May 4, 2011·Antimicrobial Agents and Chemotherapy·Markus A ZeitlingerUrsula Theuretzbacher
Feb 2, 2013·Bioorganic & Medicinal Chemistry Letters·Imran AhmadAtul Gupta
Jul 11, 2013·International Journal of Molecular Sciences·Hongliang XuZhengqiang Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.