PMID: 1204655Dec 15, 1975Paper

Luminescence studies of saccharide binding to wheat germ agglutinin (lectin)

European Journal of Biochemistry
J P Privat, M Monsigny

Abstract

The fluorescence and phosphorescence emission of wheat germ agglutinin are reported. Fluorescent tryptophan residues of wheat germ agglutinin are found highly exposed to solvent: fluorescence quenching induced by temperature fits with a single Arrhenius critical energy close to that of tryptophan in solution; the whole fluorescence emission is susceptible to iodide ion quenching and data reveal the homogeneity of fluorescence arising from only one type of tryptophan exposition. Energy transfers are analyzed at singlet and triplet state level. Tyrosine fluorescence at 25 degrees C is very weak. Results obtained from the relative excitation fluorescence quantum yield and from intrinsic fluorescence polarization show that a large amount of energy absorbed by tyrosine at 280 nm is transferred to tryptophan residues. However, tyrosine fluorescence is highly increased at 70 degrees C although disulfide bridges are not reduced. The phosphorescence spectrum at 77 K in 50% ethylene glycol is finely structured with several resolved vibrational bands at 405, 432 and 455 nm. Phosphorescence decay can be fitted with a single exponential. Lifetime is independent of excitation wave-length. Its value is very close to that of free tryptophan. I...Continue Reading

References

Aug 25, 1974·Journal of Molecular Biology·C S Wright
Aug 25, 1974·Journal of Molecular Biology·C S WrightM M Burger
Aug 15, 1974·European Journal of Biochemistry·J P PrivatM Monsigny
Jan 1, 1973·The Biochemical Journal·A K AllenN Sharon
Dec 19, 1973·Biochemical and Biophysical Research Communications·R Lotan, N Sharon
Jul 10, 1974·Biochemical and Biophysical Research Communications·R H Rice, M E Etzler
Jun 23, 1970·Biochemistry·W B De Lauder, P Wahl
Oct 1, 1970·Archives of Biochemistry and Biophysics·I Matsumoto, T Osawa
Jul 1, 1968·Biochemistry·I Weinryb, R F Steiner
Jul 16, 1962·Biochimica Et Biophysica Acta·J A GALLY, G M EDELMAN

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Citations

Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M MonsignyC Hélène
Sep 29, 2011·Biophysical Journal·María Del Carmen Portillo-TéllezEnrique García-Hernández
Mar 1, 1981·Archives of Biochemistry and Biophysics·F JordanP W Woo
Sep 15, 1976·European Journal of Biochemistry·J P PrivatJ C Auchet
Sep 7, 1984·Biochimica Et Biophysica Acta·P MidouxM Monsigny
Dec 1, 1984·Archives of Biochemistry and Biophysics·F RicchelliB Salvato
Jul 31, 1980·Biochemical and Biophysical Research Communications·P J Stein, K C Heehn

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