Luminescent and paramagnetic properties of nanoparticles shed light on their interactions with proteins

Scientific Reports
Giuditta Dal CortivoN Helge Meyer

Abstract

Nanoparticles have been recognized as promising tools for targeted drug-delivery and protein therapeutics. However, the mechanisms of protein-nanoparticle interaction and the dynamics underlying the binding process are poorly understood. Here, we present a general methodology for the characterization of protein-nanoparticle interaction on a molecular level. To this end we combined biophysical techniques including nuclear magnetic resonance (NMR), circular dichroism (CD), resonance energy transfer (RET) and surface plasmon resonance (SPR). Particularly, we analyzed molecular mechanisms and dynamics of the interaction of CaF2 nanoparticles with the prototypical calcium sensor calmodulin (CaM). We observed the transient formation of an intermediate encounter complex involving the structural region linking the two domains. Specific interaction of CaM with CaF2 NPs is driven by the N-terminal EF-hands, which seem to recognize Ca2+ on the surface of the nanoparticle. We conclude that CaF2 NP-CaM interaction is fully compatible with potential applications in nanomedicine. Overall, the methods presented in this work can be extended to other systems and may be useful to quantitatively characterize structural and dynamic features of prot...Continue Reading

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Citations

Sep 4, 2018·Frontiers in Molecular Neuroscience·Rosario ValloneDaniele Dell'Orco
Nov 1, 2019·Israel Journal of Chemistry·Y Randika PereraNicholas C Fitzkee
Jun 8, 2021·Frontiers in Microbiology·Radha P SomarathneNicholas C Fitzkee

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Methods Mentioned

BETA
protein folding
nuclear
circular
surface plasmon resonance
NMR
circular dichroism
chip
nuclear magnetic resonance
by

Software Mentioned

SPR
CCPNmr
NMRPipe

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