PMID: 20631437Jul 16, 2010Paper

Luminol encapsulated liposome as a signal generator for the detection of specific antigen-antibody reactions and nucleotide hybridization

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Pakavadee RakthongKavi Ratanabanangkoon

Abstract

Liposomes prepared with biotinylated phospholipids and luminol entrapped were shown to be of 187 nm in size, 59% of which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a longer hydrophilic PEG2000 spacer, but not with the shorter hydrophobic caproyl one, bound efficiently and specifically with immobilized streptavidin in a microplate assay. The interactions of dinitrophenol and tobramycin with their respective antibodies, and the hybridization of 20-mers oligonucleotides were studied using the liposome as a signal generator. These reactions were shown to be specific with limits of detection of 0.58 microM, 0.96 microM and 18 nM, respectively.

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