Abstract
A luminol-enhanced chemiluminescence (CL) assay was developed to evaluate type III group B streptococcal opsonins. Sera from 11 adults that contained high (greater than 25 microgram/ml), intermediate (3-24 microgram/ml), or low (less than 2 microgram/ml) antibody concentrations were assessed by CL, indirect immunofluorescence (IF), and bactericidal assays. Total CL activity correlated with antibody concentration (P less than 0.005), IgG- and C3-IF values, and phagocytic and bactericidal indices. Heat-stable CL activity correlated with antibody concentration (P less than 0.0001) and was related to IgG-IF values and phagocytic indices. Significant bactericidal activity was demonstrated in all sera containing greater than 2 microgram of antibody/ml, but no bactericidal activity was observed in heat-inactivated sera. Adsorption of heated sera with type III group B Streptococcus abolished CL activity, whereas CL values of MgEGTA-chelated sera were approximately 80% of untreated sera. Thus, type-specific antibody in addition to heat-labile serum factors, including those of the alternative complement pathway, contribute to opsonization of type III group B Streptococcus.
Citations
Nov 1, 1987·Journal of Clinical Immunology·S Bender, S Hetherington
Jun 1, 1982·Klinische Wochenschrift·J E MenitoveR H Aster
Feb 1, 1984·Theriogenology·A C AsburyG W Foster
Jan 1, 1985·Developmental and Comparative Immunology·A L ScottP H Klesius
May 1, 1982·Annales d'immunologie·B Descamps-LatschaM N Feuillet-Fieux
Mar 12, 1981·The New England Journal of Medicine·J D Siegel, G H McCracken
Apr 1, 1995·Acta Paediatrica Japonica; Overseas Edition·A NishidaM Nakano
Apr 16, 1998·Archives of Disease in Childhood. Fetal and Neonatal Edition·J KällmanE Kihlström
Oct 1, 1981·The Journal of Clinical Investigation·D C AndersonC W Smith
May 9, 2001·Leukemia & Lymphoma·Y MaedaM Harada
Jun 1, 1983·Current Problems in Pediatrics·K L CatesM Ballow
Mar 9, 2005·Developmental and Comparative Immunology·Sami NikoskelainenEsa-Matti Lilius
May 1, 1987·Parasite Immunology·M Li, Y J Li
Jul 20, 2001·Molecular Genetics and Metabolism·M A Mazade, M S Edwards
May 8, 1997·British Journal of Nursing : BJN·B Greatrex
Dec 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·C SöderströmA G Sjöholm
Oct 1, 1989·Journal of Clinical Microbiology·Y NaganoY Okuwaki
Dec 1, 1995·Infection and Immunity·S TakahashiY Okuwaki
Mar 1, 1981·Journal of Clinical Microbiology·S L KaplanR D Feigin
Oct 1, 1987·Infection and Immunity·K M ButlerM S Edwards
Mar 1, 1982·Infection and Immunity·S E GardnerC J Baker
Feb 1, 1982·Infection and Immunity·B J De CueninckR M Swenson
Apr 1, 1993·Infection and Immunity·F J NoyaM S Edwards
Jan 1, 1981·Infection and Immunity·K K MatthayA J Ammann
Sep 1, 1982·Infection and Immunity·B J De CueninckR M Swenson
Feb 1, 1984·Infection and Immunity·M F TosiD C Anderson
Jan 1, 1983·Clinical Immunology and Immunopathology·T Tono-OkaS Matsumoto
Aug 1, 1984·Clinical Immunology and Immunopathology·T MeshulamJ D Sobel