PMID: 20126899Feb 4, 2010Paper

Lung apoptosis after intra-pulmonary instillation of Benzo(a)pyrene in Wistar rats

Acta Cirúrgica Brasileira
Baldomero Antonio Kato da SilvaRenato Silva Nacer

Abstract

To evaluate the influence of pulmonary instillation of Benzo[a]pyrene in lung apoptosis of Wistar rats. Male Rattus norvegicus albinus, Wistar lineage was carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%. Three experimental groups had been formed with 08 animals each: Control Group (Alcohol 70%); B[a]P Group 40 mg/kg; e B[a]P Group 80mg/kg, submitted to euthanasia 16 and 18 weeks after the experimental procedure. The pulmonary sections had been processed by TUNEL method and submitted to the histomorphometric analysis to quantify the apoptotic cell number. After 16 weeks, mean of apoptotic cells number in control group (19,3 + or - 3,2) was greater than 40mg/Kg group (11,8 + or - 1,9; p<0,01) and 80mg/Kg group (7,0 + or - 1,4; p<0,01). Significant difference also observed between 40mg/Kg and 80mg/Kg (p<0,05). After 18 weeks, mean of apoptotic cells number in control group (18,0 + or - 2,2) was greater than 40mg/Kg group (8,8 + or - 1,7; p<0,01) and 80mg/Kg group (5,5 + or - 1,3; p<0,01). Significant difference wasn't observed between 40mg/Kg and 80mg/Kg (ns). Intra-pulmonary instillation of Benzo[a]pyrene induces significant decrease of apoptotic activity in lung tissue.

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Citations

Jan 16, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A WannhoffT Reuther

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis