Lung cancer cells often express high levels of protein kinase C activity

Japanese Journal of Cancer Research : Gann
M HiraiN Shimizu

Abstract

We analyzed protein kinase C (PKC) activity in twenty-two tumor cell lines derived from lung, pancreas, stomach, tongue and vulva, and found that lung cancer cells often (9 out of 13) exhibit significantly higher PKC activity than other types of cancer cells. The PKC in these lung cancer cells was separated into one major and one minor peaks by a Mono Q column chromatography. The PKC in the major peak had an absolute requirement for Ca2+, phosphatidylserine and 12-O-tetradecanoylphorbol-13-acetate (TPA), as expected. However, the PKC in the minor peak did not require TPA for its activation. Hydroxyapatite column chromatography revealed that the PKC in the major peak is type III. These results indicate that in lung cancer cells type III PKC activity is often elevated compared to other types of cancer cells. The growth of many lung cancer cell lines was inhibited by TPA.

References

Jul 18, 1986·Science·Y Nishizuka
Jan 1, 1987·Molecular and Cellular Biology·J R Woodgett, T Hunter
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J E BussG N Gill

❮ Previous
Next ❯

Citations

Jun 1, 1991·Journal of in Vitro Fertilization and Embryo Transfer : IVF·Y EndoS Suzuki
Jan 23, 1999·The American Journal of Pathology·P CornfordC S Foster
Apr 1, 1995·Clinical Molecular Pathology·J M Lord, J Pongracz
May 15, 2001·Biochemical and Biophysical Research Communications·S S PandianS D Heys
Sep 1, 1992·Journal of Cellular Physiology·Y LinE W Gabrielson
Dec 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J SzalayR Vazeos
Feb 15, 1994·International Journal of Cancer. Journal International Du Cancer·C StanwellG R Pettit
Apr 22, 1992·International Journal of Cancer. Journal International Du Cancer·T D BradshawG R Pettit
Jan 6, 2001·Scandinavian Journal of Urology and Nephrology. Supplementum·C S FosterW A Sakr
Mar 25, 2010·Journal of Cell Science·Hsin-Yuan FangKuan-Chih Chow
Jun 3, 2021·International Journal of Molecular Sciences·Mohammad Mojtaba SadeghiYusuf A Hannun
Aug 1, 1990·Medical Hypotheses·M ChigiraH Watanabe
Aug 30, 1989·Biochemical and Biophysical Research Communications·Y HashimotoT Kuroki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.