Lung deposition of droplet aerosols in monkeys

Inhalation Toxicology
Y S ChengC H Hobbs

Abstract

Nonhuman primates are often the animal models of choice to study the infectivity and therapy of inhaled infectious agents. Most animal models for inhaled infectious diseases use aerosol/droplets generated by an atomization technique such as a Collison nebulizer that produces particles in the size range of 1 to 3 microm in diameter. There are few data in the literature on deposition patterns in monkeys. Our study was designed to measure the deposition pattern in monkeys using droplets having diameters of 2 and 5 microm using an exposure system designed to expose monkeys to aerosols of infectious agents. Six cynomolgus monkeys were exposed to droplets. The aerosol solution was generated from a Vero cell supernate containing DMEM + 10% fetal bovine serum tagged with Tc-99m radiolabel. Collison and Retec nebulizers were used to generate small and large droplets, respectively. The particle size (as determined from a cascade impactor) showed an activity median aerodynamic diameter (AMAD) of 2.3 and 5.1 microm for the Collison and Retec nebulizer, respectively. The animals were anesthetized, placed in a plethysmography box, and exposed to the aerosol. The deposition pattern was determined using a gamma camera. Deposition in the head a...Continue Reading

References

Jan 1, 1985·Journal of Toxicology and Environmental Health·R B Schlesinger
Jan 1, 1993·European Journal of Nuclear Medicine·N I ForgeM J O'Doherty
Dec 14, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·G M ZauchaL Hensley
Dec 6, 2003·American Journal of Respiratory and Critical Care Medicine·Kevin P FennellyLeonid B Heifets
Mar 19, 2005·Pediatric Research·Jean C DubusPatrice Diot
May 4, 2005·Environmental Health Perspectives·Yung Sung ChengDan G Baden
Jul 1, 1947·The American Journal of Physiology·A C GUYTON

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Citations

Jan 6, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jeffry D SchroeterHarvey J Clewell
Apr 14, 2010·Infection and Immunity·Frederick KosterC R Lyons
Feb 25, 2011·PLoS Neglected Tropical Diseases·Robert Colby LaytonFrederick Koster
Apr 24, 2012·Trends in Microbiology·Kortney M GustinTaronna R Maines
Aug 21, 2010·Journal of Medical Primatology·R Colby LaytonFrederick Koster
Jul 15, 2015·The Journal of Clinical Investigation·Michelle MeyerAlexander Bukreyev
Jan 15, 2011·International Journal of Pharmaceutics·Thiago C CarvalhoRobert O Williams
Aug 5, 2010·Veterinary Pathology·C J RoyJ A Hutt
Jun 9, 2018·American Journal of Respiratory and Critical Care Medicine·Daniel LightwoodRoger T Palframan
Jul 3, 2019·AAPS PharmSciTech·Philip J KuehlWesley H DeHaan
Apr 26, 2021·Molecular Immunology·Julien LemaitrePauline Maisonnasse
Jun 12, 2021·Archives of Disease in Childhood. Fetal and Neonatal Edition·Luke JardineDavid Durand
Jun 5, 2021·Applied and Environmental Microbiology·Sadegh NiaziGraham R Johnson
Dec 2, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Bailey C Basso, Joshua B Bostwick

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