Lung endothelial heparan sulfates mediate cationic peptide-induced barrier dysfunction: a new role for the glycocalyx

American Journal of Physiology. Lung Cellular and Molecular Physiology
Randal O DullJoe G N Garcia

Abstract

The endothelial glycocalyx is believed to play a major role in microvascular permeability. We tested the hypothesis that specific components of the glycocalyx, via cytoskeletal-mediated signaling, actively participate in barrier regulation. With the use of polymers of arginine and lysine as a model of neutrophil-derived inflammatory cationic proteins, we determined size- and dose-dependent responses of cultured bovine lung microvascular endothelial cell permeability as assessed by transendothelial electrical resistance (TER). Polymers of arginine and lysine >11 kDa produced maximal barrier dysfunction as demonstrated by a 70% decrease in TER. Monomers of l-arginine and l-lysine did not alter barrier function, suggesting a cross-linking requirement of cell surface "receptors". To test the hypothesis that glycosaminoglycans (GAGs) are candidate receptors for this response, we used highly selective enzymes to remove specific GAGs before polyarginine (PA) treatment and examined the effect on TER. Heparinase III attenuated PA-induced barrier dysfunction by 50%, whereas heparinase I had no effect. To link changes in barrier function with structural alterations, we examined actin organization and syndecan localization after PA. PA ind...Continue Reading

References

May 1, 1990·The Journal of Clinical Investigation·H A PereiraJ K Spitznagel
Dec 1, 1989·Journal of Cellular Physiology·M R PowersA B Malik
Jul 15, 1988·Biochemical and Biophysical Research Communications·D E Humphries, J E Silbert
Jul 1, 1980·Microvascular Research·F E Curry, C C Michel
Aug 1, 1995·The American Journal of Physiology·G SaumonG Martet
Dec 9, 1993·Nature·I Giaever, C R Keese
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H A PereiraJ K Spitznagel
Apr 1, 1997·The American Journal of Physiology·S VepaV Natarajan
May 1, 1997·American Journal of Respiratory Cell and Molecular Biology·J G GarciaA D Verin
Jul 1, 1997·The American Journal of Physiology·J G GarciaC E Patterson
Oct 29, 1998·The Journal of Biological Chemistry·J K LangfordR D Sanderson
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SaoncellaP F Goetinck
Jun 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Zimmermann, G David
Oct 8, 1999·The Journal of Clinical Investigation·A M OlofssonE Lundgren-Akerlund
Oct 21, 1999·Microvascular Research·X Hu, S Weinbaum
Feb 24, 2000·The Journal of Pathology·H A LehrC J Kirkpatrick
May 10, 2000·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·C W Lush, P R Kvietys
Sep 28, 2000·Pflügers Archiv : European journal of physiology·A R PriesP Gaehtgens
Jan 3, 2001·Nature Medicine·N KeiserR Sasisekharan
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·V NatarajanN L Parinandi
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A D VerinJ G Garcia
Sep 25, 2001·Journal of Applied Physiology·S M Dudek, J G Garcia
May 16, 2002·The Journal of Cell Biology·Arie HorowitzMichael Simons
Jun 28, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joe G N GarciaAlexander D Verin
Dec 20, 2002·Angewandte Chemie·Ishan Capila, Robert J Linhardt

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Citations

Nov 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mia M ThiDavid C Spray
May 8, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andrew P StevensRandal O Dull
May 21, 2010·The Journal of Clinical Investigation·Dianhua JiangPaul W Noble
Oct 21, 2011·Physiology·Eric P SchmidtGregory P Downey
Feb 13, 2007·Biochemical and Biophysical Research Communications·Manolis Y PahakisJohn M Tarbell
Oct 29, 2010·Blood·Anna KlinkeStephan Baldus
Jul 13, 2006·Annual Review of Biomedical Engineering·Ram SasisekharanVikas Prabhakar
Jun 7, 2014·Annual Review of Biomedical Engineering·John M TarbellFitz-Roy E Curry
Sep 13, 2016·Journal of Intensive Care·Akira UshiyamaTakehiko Iijima
Oct 6, 2016·Intensive Care Medicine Experimental·Peter BentzerAdam Linder
Jan 13, 2018·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Inkyung KangCharles W Frevert
May 25, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Srikar R GuntakaRadhakrishna Rao
Jul 27, 2004·Journal of Applied Physiology·Randal O DullClara Fronticelli
Mar 28, 2018·American Journal of Respiratory Cell and Molecular Biology·Sarah M HaegerEric P Schmidt
Oct 18, 2003·Circulation Research·Jeffry A FlorianJohn M Tarbell
Jul 9, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Sashka DimitrievskaLaura E Niklason
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Oct 12, 2011·Inflammatory Bowel Diseases·A M PattersonD Kelly
Jul 10, 2013·Anesthesia and Analgesia·Stephen R CollinsRandal O Dull
Jul 30, 2014·Tissue Barriers·Shravan Kumar SriramanVladimir P Torchilin
Nov 9, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dolly MehtaWolfgang M Kuebler
Oct 11, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Aubrey E Taylor
Aug 24, 2004·Journal of Applied Physiology·Kristy M AinslieJohn M Tarbell
Aug 11, 2010·Bioconjugate Chemistry·Lolita ZaliauskieneSarunas Zigmantas
Dec 5, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Heidi N du PreezJohnson Lin

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