PMID: 9635248Jun 23, 1998Paper

Lung injury and degradation of extracellular matrix components by Aspergillus fumigatus serine proteinase

Experimental Lung Research
P IadarolaM Luisetti

Abstract

Aspergillus fumigatus produces a variety of extracellular proteinases that are believed to be virulence factors towards Aspergillus-related lung disease. Among Aspergillus proteinases, the serine proteinase is thought to play a major virulent role because of its widespread production. Nevertheless, evidence of direct pulmonary injury caused by the A. fumigatus serine proteinase is still lacking. The purpose of our work was: (1) to provide evidence for a pivotal role of A. fumigatus serine proteinase in producing lung injury in an animal model, and (2) to investigate the broadness of the substrate specificity of the proteinase towards extracellular matrix components. To achieve this aim, the proteinase from an A. fumigatus strain isolated from human airways was purified by a four-step procedure, including cation exchange and hydrophobic interaction. High-performance capillary electrophoresis, SDS-PAGE, determination of K(m) towards synthetic substrates, and inhibitory studies were used to further characterize the A. fumigatus serine proteinase. With reference to extracellular matrix components, the A. fumigatus serine proteinase was shown to degrade human lung elastin at a higher rate than an equimolar amount of human neutrophil...Continue Reading

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Citations

Dec 11, 1999·Mycoses·R Rüchel
Jul 15, 2009·Clinical Microbiology Reviews·Taylor R T Dagenais, Nancy P Keller
Feb 12, 2009·Mycoses·Suganthini KrishnanPranatharthi H Chandrasekar
Apr 14, 2015·Nature Communications·Nariman A BalengaKirk M Druey
Aug 18, 2006·Biological Chemistry·Jonathan L MoonJames Travis
May 5, 2017·Annals of Thoracic Medicine·Ibrahim Ahmed JanahiAmal Rashid Al-Naimi
Mar 27, 2001·Respirology : Official Journal of the Asian Pacific Society of Respirology·P A Wark, P G Gibson
Apr 21, 2004·Journal of Medical Microbiology·Yoshiyuki OkumuraToshiaki Nikai
Jul 3, 2019·Canadian Journal of Microbiology·Zhiping HanHelena Nevalainen
Sep 27, 2005·Veterinary Immunology and Immunopathology·Terhi Simonen-JokinenTimo Sorsa

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