Abstract
An early phase of proliferation by mesothelial cells and fibroblasts occurs in the lung after asbestos deposition, but the source and identity of the cytokine(s) involved is not clear. In the present study, rats received crocidolite asbestos intratracheally and were killed at 1 and 6 wk later, animals received tritiated thymidine 1 h before death. An increase in inflammatory cells was found in bronchoalveolar and pleural lavage fluids at both times, and after 6 wk the lungs showed fibrosis which was confirmed biochemically. Asbestos fibers were found in alveolar and interstitial macrophages but not in pleural macrophages. In autoradiographs, both mesothelial cells and fibroblasts showed increased DNA synthesis at 1 wk, but only fibroblast labeling was increased at 6 wk. Lavaged macrophages from the lung (alveolar macrophages; AM) and the pleura (pleural macrophages; PLM) were cultured and supernatants tested on rat lung mesothelial cells and fibroblasts in vitro; concentrated lavaged fluids were also tested. At 1 wk, macrophages secreted growth factors for both cell types but the effect was more pronounced using lavage fluids, particularly from the pleura. The increase in fibroblast growth was reduced by an antibody to platelet...Continue Reading
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