PMID: 8586071Oct 1, 1995Paper

Lung tumor cells: a multivariate approach to cell classification using two-dimensional protein pattern

Electrophoresis
H R SchmidD Vonderschmitt

Abstract

High resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is a powerful research tool for the analytical separation of cellular proteins. The qualitative and quantitative pattern of polypeptides synthesized by a cell represents its phenotype and thus defines characteristics such as the morphology and the biological behavior of the cell. By analyzing and comparing the protein patterns of different cells it is possible to recognize the cell type and also to identify the most typical features of these cells. In applied pathology it is often difficult to identify the tissue of origin and the stage or grade of a neoplasia by cellular morphology analyzed by classical or immunostaining procedures. The protein pattern itself is the most characteristic feature of a cell and should therefore contribute to the identification of the cell type. For this reason we separated protein fractions originating from different lung tumor cell lines using 2-D PAGE and we compared the resulting patterns on a multivariate statistical level using correspondence analysis (CA) and ascendant hierarchical clustering (AHC). The results indicate that (i) protein patterns are highly typical for cells and that (ii) the comparison of the prote...Continue Reading

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Citations

May 8, 2000·International Journal of Cancer. Journal International Du Cancer·A A AlaiyaG Auer
Feb 8, 2008·Analytical and Bioanalytical Chemistry·Emilio MarengoPier Giorgio Righetti
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