Lupeol inhibits LPS-induced NF-kappa B signaling in intestinal epithelial cells and macrophages, and attenuates acute and chronic murine colitis

Life Sciences
Changhyun LeeJoo Sung Kim

Abstract

Lupeol, a natural pentacyclic triterpene, exhibits anti-inflammatory effects. However, its role in colitis has not been investigated. In the present study, we evaluated the effect of lupeol on the NF-κB signaling pathway and experimental colitis in mice. The human intestinal epithelial cells (IECs) COLO 205 and the murine macrophages RAW 264.7 were pretreated with lupeol and then stimulated with lipopolysaccharide (LPS). The production of inflammatory cytokines (IL-8 from COLO 205; IL-6, IL-12 and TNF-α from RAW 264.7) was determined by ELISA. The effect of lupeol on NF-κB pathway was examined by Western blot analysis of IκBα phosphorylation/degradation and an electrophoretic mobility shift assay (EMSA). For in vivo studies, dextran sulfate sodium (DSS)-induced acute colitis model and chronic colitis model in IL-10(-/-) mice were used. Colitis was quantified by disease activity index, colon length and histologic evaluation. Lupeol strongly suppressed pro-inflammatory cytokine production in IECs and murine macrophages. It also inhibited LPS-induced IκBα phosphorylation/degradation and the DNA binding activity of NF-κB. The oral administration of lupeol significantly reduced the colitis activity and histologic scores in both acut...Continue Reading

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Citations

Sep 8, 2016·Pharmaceutical Biology·Yadu Nandan DeyAnkush D Jadhav
Feb 8, 2019·Critical Reviews in Food Science and Nutrition·Imam HossenXiao Junsong
May 23, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S C X de-AlmeidaG S de-Barros Viana
Dec 15, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Kai LiuJia Luo
Mar 9, 2021·Expert Opinion on Therapeutic Targets·Yaoyao DuBing Wang
Mar 5, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Kyle DriscollWusirika Ramakrishna
Jun 23, 2020·International Immunopharmacology·Eun Ae KangJoo Sung Kim
Jul 30, 2021·Journal of Ethnopharmacology·Camila Helena BarrosDênia Antunes Saúde-Guimarães
Sep 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Cibele Rodrigues ToledoArmando Silva-Cunha

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