Lutein prevents osteoarthritis through Nrf2 activation and downregulation of inflammation

Archives of Medical Science : AMS
Yan-Qin QiaoYan-Zheng Gao

Abstract

Osteoarthritis is an inflammatory disorder associated with oxidative stress and apoptosis leading to cartilage destruction and impairment of cartilage formation. In the present study, we studied the protective effect of lutein against monosodium iodoacetate (MIA)-induced osteoarthritis in primary chondrocyte cells. Oxidative stress was determined through testing antioxidant status, reactive oxygen species levels and lipid peroxide content. Also, Nrf2 expression and its downstream target genes HO-1 and NQO-1 were determined. Inflammation was analyzed through NF-κB, COX-2 and pro-inflammatory cytokines (IL-6, TNF-α, IL-1β). In addition, the effects of MIA and lutein on mitochondrial membrane potential and caspase-3 levels were analyzed. The results showed that lutein treatment significantly increased the cell viability of chondrocytes and offered significant cytoprotection by enhancing the antioxidant defense mechanisms and reducing oxidative stress (reactive oxygen species and lipid peroxidation). Lutein treatment showed anti-inflammatory effects by downregulating inflammatory proteins (NF-κB, COX-2) and pro-inflammatory cytokines (IL-6, TNF-α, IL-1β). Lutein reduced MIA-induced apoptosis through maintaining mitochondrial membra...Continue Reading

Citations

Mar 3, 2020·Phytotherapy Research : PTR·Cong LuFengzhong Wang
Aug 17, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Minh Anh Thu PhanJayashree Arcot
Oct 24, 2020·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Guifang ChenYuxia Ma
Nov 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bei CuiYuanyuan Guo
Mar 12, 2021·The Journal of Nutritional Biochemistry·Shweta SharmaSubhrajyoti Chatterjee
May 11, 2021·Reports of Biochemistry & Molecular Biology·Noha El-Sayed IbrahimMarwa Abdelgwad

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