PMID: 7522739Jun 1, 1994Paper

Luteinizing hormone-releasing hormone receptor messenger ribonucleic acid expression in the rat pituitary during lactation and the estrous cycle

Journal of Neuroendocrinology
T FunabashiD W Pfaff

Abstract

To study mechanisms underlying the modulation of luteinizing hormone-releasing hormone receptor (LHRH-R) during lactation and the estrous cycle, we used a reverse transcriptase-polymerase chain reaction (RT-PCR) procedure to generate a probe for rat LHRH-R messenger RNA (mRNA). Using primers based on the mouse sequence, we amplified an approximately 300 bp fragment from rat pituitary complementary DNA. This PCR product was shown to be part of LHRH-R cDNA by direct sequencing and by comparing to the rat LHRH-R cDNA reported recently. Then, this PCR fragment was used as a probe for northern blotting analysis. The level of LHRH-R mRNA in the pituitary was significantly decreased during lactation, by approximately 80%, compared to that of ovariectomized and intact (diestrous and metestrous cycling) rats while no statistical difference in glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) mRNA level was observed between groups. During the estrous cycle, the level of LHRH-R mRNA in the pituitary was about two-fold higher on diestrous day 2 and the morning of proestrus than that on diestrous day 1 and quickly returned toward control level by noon of proestrus. In addition, we found that GAPDH mRNA levels from a so-called housekeeping ge...Continue Reading

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Nov 1, 1992·Brain Research. Molecular Brain Research·P J BrooksD W Pfaff
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Citations

Jan 1, 1995·Brain Research. Molecular Brain Research·T FunabashiD W Pfaff
Jun 1, 1996·Brain Research. Molecular Brain Research·V Quiñones-JenabD W Pfaff
Aug 16, 2012·Endocrinology·Zahara AlimUlrich Boehm
Jul 11, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joseph R KurianAnthony P Auger
Apr 29, 2008·Journal of Chemical Neuroanatomy·Asher J AlbertsonDonal C Skinner
Feb 4, 2009·Journal of Neuroendocrinology·D C SkinnerN Scanlan-Blake
Nov 26, 2013·Brain Research·Robin M Forbes-LormanAnthony P Auger
Feb 12, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G V Childs, G Unabia
Nov 15, 1997·Microscopy Research and Technique·G V Childs
Apr 22, 2005·Journal of Neuroendocrinology·K M Olesen, A P Auger

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