Luteolin protects PC-12 cells from H2 O2 -induced injury by up-regulation of microRNA-21

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Zhiti ZhangLei Shi

Abstract

Ischemic cerebrovascular disease (ICVD) is the third leading cause of death worldwide. Luteolin is a naturally flavonoid widely distributed in many plant leaves. This study aimed to explore the effects of luteolin on H2O2-induced ICVD cell oxidative injury model, as well as underlying molecular mechanisms. Viability and apoptosis of PC-12 cells and rat brain microvascular endothelial cells (rBMECs) were detected using CCK-8 assay and FITC-Annexin V/PI staining, respectively. The levels of ROS and MDA were measured using DCFH-DA staining and MDA assay kit, respectively. Cell transfection was conducted to change the expression level of miR-21. Expression levels of key factors involved in cell proliferation, oxidative stress, apoptosis, PI3K/AKT and PDCD4/p21 pathways were evaluated using western blotting. Low concentration of luteolin had no significant effect on PC-12 cell viability and presented protective effects on H2O2-induced PC-12 cell viability loss, proliferation inhibition, ROS generation, oxidative stress increase and apoptosis. Moreover, luteolin up-regulated the expression level of miR-21 in H2O2-treated PC-12 cells. Overexpression of miR-21 strengthened the protective effects of luteolin on H2O2-induced PC-12 cell o...Continue Reading

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Citations

Aug 2, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Guanglei GaoShengcui Liu
Feb 14, 2020·Artificial Cells, Nanomedicine, and Biotechnology·Dan Deng, Hui Liang
May 15, 2019·Cells·Edoardo ParrellaMarina Pizzi
Mar 16, 2021·Journal of Ethnopharmacology·Zahra Tayarani-NajaranBehjat Javadi

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